国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
1期
1-3
,共3页
IL-10%人表皮细胞%DNA%荧光强度
IL-10%人錶皮細胞%DNA%熒光彊度
IL-10%인표피세포%DNA%형광강도
IL-10%Human epidermal cells%DNA%Fluorescence intensity
目的 探讨IL-10对人表皮细胞增殖分化过程中DNA含量的影响.方法 将正常人皮肤移植于裸鼠,建立人皮肤-裸鼠嵌合模型60只;按配对设计要求随机分成实验组和对照组各30只,实验组使用IL-10 0.1 ml (10 ng/ml)注射于裸鼠移植皮肤内,对照组使用0.9％氯化钠0.1ml注射于裸鼠移植皮肤内,均每天1次、连续使用28天,最后以激光扫描共聚焦显微镜测试移植皮肤人表皮细胞DNA荧光强度值.结果 IL-10可使人表皮细胞DNA荧光强度值显著降低[实验组(353 928.93±11 672.33) vs.对照组(369 649.30±8576.48),t=5.97,P＜0.05].结论 IL-10能减少人表皮细胞增殖分化过程中DNA含量.
目的 探討IL-10對人錶皮細胞增殖分化過程中DNA含量的影響.方法 將正常人皮膚移植于裸鼠,建立人皮膚-裸鼠嵌閤模型60隻;按配對設計要求隨機分成實驗組和對照組各30隻,實驗組使用IL-10 0.1 ml (10 ng/ml)註射于裸鼠移植皮膚內,對照組使用0.9％氯化鈉0.1ml註射于裸鼠移植皮膚內,均每天1次、連續使用28天,最後以激光掃描共聚焦顯微鏡測試移植皮膚人錶皮細胞DNA熒光彊度值.結果 IL-10可使人錶皮細胞DNA熒光彊度值顯著降低[實驗組(353 928.93±11 672.33) vs.對照組(369 649.30±8576.48),t=5.97,P＜0.05].結論 IL-10能減少人錶皮細胞增殖分化過程中DNA含量.
목적 탐토IL-10대인표피세포증식분화과정중DNA함량적영향.방법 장정상인피부이식우라서,건립인피부-라서감합모형60지;안배대설계요구수궤분성실험조화대조조각30지,실험조사용IL-10 0.1 ml (10 ng/ml)주사우라서이식피부내,대조조사용0.9％록화납0.1ml주사우라서이식피부내,균매천1차、련속사용28천,최후이격광소묘공취초현미경측시이식피부인표피세포DNA형광강도치.결과 IL-10가사인표피세포DNA형광강도치현저강저[실험조(353 928.93±11 672.33) vs.대조조(369 649.30±8576.48),t=5.97,P＜0.05].결론 IL-10능감소인표피세포증식분화과정중DNA함량.
Objective To study the impact of IL-10 on the content of DNA during the proliferation and differentiation of human epidermal cells.Methods The normal human skin was transplanted to nude mice to establish a skin-nude mice chimeric model in 60 mice.According to requirements in matched-pairs design,the mice were randomly divided into a study group and a control group,30 cases for each group.In the study group,IL-10 of 0.1ml (10 ng/ml) was injected into the skin graft,while 0.9％ sodium chloride of 0.1 ml was injected into the skin graft once daily for 28 days.The value of DNA fluorescence intensity of human epidermal cells was detected with a laser scanning confocal microscope.Result IL-10 markedly reduced the value of DNA fluorescence intensity of human epidermal cells (t=5.97,P ＜ 0.05).Conclusion IL-10 can reduce the content of DNA during the proliferation and differentiation of human epidermal cells.
