CAJ | 학술논문

目的建立清肺丸的质量标准。方法采用薄层色谱法对制剂中西青果、山豆根进行定性鉴别；采用高效液相色谱法对该制剂中栀子的有效成分栀子苷进行含量测定,选择Agilent Eclipse XDB-C18(150 mm×4.6 mm,5μm)色谱柱,乙腈-水(10∶90)为流动相,流速1.0 mL/min,柱温为室温,检测波长为238 nm。结果西青果、山豆根的薄层鉴别简便快速、重复性好。栀子苷在0.238～3.808μg范围呈良好的线性关系,平均回收率为97.24%,RSD＝1.60%(n＝6)。结论本法专属性强,重复性好,可以有效控制清肺丸的质量。
목적건립청폐환적질량표준。방법채용박층색보법대제제중서청과、산두근진행정성감별；채용고효액상색보법대해제제중치자적유효성분치자감진행함량측정,선택Agilent Eclipse XDB-C18(150 mm×4.6 mm,5μm)색보주,을정-수(10∶90)위류동상,류속1.0 mL/min,주온위실온,검측파장위238 nm。결과서청과、산두근적박층감별간편쾌속、중복성호。치자감재0.238～3.808μg범위정량호적선성관계,평균회수솔위97.24%,RSD＝1.60%(n＝6)。결론본법전속성강,중복성호,가이유효공제청폐환적질량。
Objective To set up the quality standard of TCM preparation Qingfei pill. Methods TLC methods were used to the qualitative identification of Fructus Termininaliae Immaturus and Radix Sophorae Subprostratae in the preparation. The content of Gardenoside, the effective component of Fructus Gardeniae, was determined by HPLC method, using Agilent Eclipse XDB-C18 (150 mm×4.6 mm, 5 μm) and acetonitrile-water (10∶90) as mobile phase with flow rate at 1.0 mL/min. The column temperature was room temperature, and the detection wavelength was 238 nm. Results The TLC methods for Fructus Termininaliae Immaturus and Radix Sophorae Subprostratae identifications were simple and rapid, and showed good repetitiveness. Gardenoside presented a good linear relationship within the range of 0.238-3.808 μg, and the average recovery rate was 97.24%, RSD was 1.60% (n=6). Conclusion These methods showed strong specificity and good repetitiveness, and can be used to control the quality of the product effectively.