CAJ | 학술논문

目的建立生新发胶囊的质量标准,为其质量控制提供依据。方法采用薄层色谱法对生新发胶囊中的制何首乌、补骨脂、天麻进行定性鉴别,采用高效液相色谱法对生新发胶囊中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷进行含量测定。采用Agilent TC-C18柱(4.6 mm×250 mm,5μm),乙腈-水(15∶85)为流动相,流速1.0 mL/min,柱温为30℃,检测波长320 nm。结果薄层色谱中斑点清晰,2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷0.05～0.50μg 范围内呈良好的线性关系,平均回收率为97.8%,RSD＝0.39%。结论该方法可行,结果准确,可用于生新发胶囊的质量控制。
목적건립생신발효낭적질량표준,위기질량공제제공의거。방법채용박층색보법대생신발효낭중적제하수오、보골지、천마진행정성감별,채용고효액상색보법대생신발효낭중2,3,5,4’-사간기이분을희-2-O-β-D-포도당감진행함량측정。채용Agilent TC-C18주(4.6 mm×250 mm,5μm),을정-수(15∶85)위류동상,류속1.0 mL/min,주온위30℃,검측파장320 nm。결과박층색보중반점청석,2,3,5,4’-사간기이분을희-2-O-β-D-포도당감0.05～0.50μg 범위내정량호적선성관계,평균회수솔위97.8%,RSD＝0.39%。결론해방법가행,결과준학,가용우생신발효낭적질량공제。
Objective To establish the quality standard for Shengxinfa Capsule. Methods TLC was used to identify Praeparata, Psoraleae Fructus and Gastrodiae Rhizoma in Shengxinfa Capsule, and 2,3,5,4’-the four-stilbene-2-O-β-D-glucoside was identified by HPLC. The column of Agilent TC-C18 (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (15∶85). The flow was 1.0 mL/min and column temperature was 30 ℃. UV detecting wavelength was 320 nm. Results Praeparata, Psoraleae Fructus and Gastrodiae Rhizoma could be identified by TLC. The linear range of 2,3,5,4’-the four-stilbene-2-O-β-D-glucoside was in the range of 0.05-0.50 μg. The average recovery was 97.8% (RSD=0.39%). Conclusion The method is feasible and accurate. The quality of Shengxinfa Capsule can be controlled effectively with the established quality standard.