CAJ | 학술논문

目的从分子水平探讨寿胎丸安胎的作用机制。方法 CBA/J×BALB/C建立正常妊娠模型,CBA/J×DBA/2建立复发性流产模型,将复发性流产模型CBA/J×DBA/2孕鼠按怀孕先后顺序随机分为模型组和寿胎丸高、中、低剂量组,从妊娠第1日开始灌胃给药,至孕14 d处死小鼠,采用免疫组化方法检测差异蛋白质热休克蛋白27、α-烯醇化酶、转铁蛋白、膜联蛋白 A2蛋白表达。结果与正常组比较,模型组子宫蜕膜热休克蛋白27、α-烯醇化酶表达明显升高,转铁蛋白、膜联蛋白A2表达明显降低,差异有统计学意义(P＜0.01)；与模型组比较,寿胎丸高、中、低剂量组蜕膜热休克蛋白27、α-烯醇化酶表达均降低,膜联蛋白A2表达均升高,寿胎丸高剂量组子宫蜕膜转铁蛋白表达升高,差异有统计学意义(P＜0.01)；寿胎丸中、低剂量组蜕膜转铁蛋白表达差异无统计学意义(P＞0.05)。结论寿胎丸通过上述蛋白的表达,从而实现维持妊娠,降低胚胎丢失率,这可能是其安胎的作用机制之一。
목적종분자수평탐토수태환안태적작용궤제。방법 CBA/J×BALB/C건립정상임신모형,CBA/J×DBA/2건립복발성유산모형,장복발성유산모형CBA/J×DBA/2잉서안부잉선후순서수궤분위모형조화수태환고、중、저제량조,종임신제1일개시관위급약,지잉14 d처사소서,채용면역조화방법검측차이단백질열휴극단백27、α-희순화매、전철단백、막련단백 A2단백표체。결과여정상조비교,모형조자궁세막열휴극단백27、α-희순화매표체명현승고,전철단백、막련단백A2표체명현강저,차이유통계학의의(P＜0.01)；여모형조비교,수태환고、중、저제량조세막열휴극단백27、α-희순화매표체균강저,막련단백A2표체균승고,수태환고제량조자궁세막전철단백표체승고,차이유통계학의의(P＜0.01)；수태환중、저제량조세막전철단백표체차이무통계학의의(P＞0.05)。결론수태환통과상술단백적표체,종이실현유지임신,강저배태주실솔,저가능시기안태적작용궤제지일。
Objective To explore the mechanism action of Shoutai Pill in the embryo from the molecular level. Methods The model of normal pregnancy was established with the model of recurrent abortion CBA/J ×DBA/2. The recurrent abortion model CBA/J×DBA/2 in pregnant mice were randomly divided into model group, high-, medium-, low-dose group of Shoutai Pill. From the first day of gestation, mice were given medicine by gavage for 14 d, and then sacrificed. Immunohistochemistry was used to detect differences in protein HSP27,α-enolase, transferrin, annexin A2 protein expression. Results Compared with normal group, decidual HSP27 and α-enolase expression of model group increased significantly, the expression of transferrin and annexin A2 was significantly decreased, with significant differences (P<0.01). Compared with model group, decidual HSP27 andα-enolase expression of Shoutai Pill high and low dose group decreased, annexin A2 expression was increased. Compared with model group, expression of decidual transferrin of Shoutai Pill high dose group increased, with significant differences (P<0.01), decidual transferrin expression of Shoutai Pill medium and low dose group had no significant difference (P>0.05). Conclusion Through the protein expression, Shoutai Pill achieves the maintenance of pregnancy, reducing the rate of embryo resorption, which may be one of the mechanisms of Shoutai Pill preventing miscarriage effect.