CAJ | 학술논문

目的观察心肌梗死大鼠毛细血管周细胞变化及益气活血中药的作用，探讨益气活血中药改善心肌血流灌注的作用机制。方法采用SD大鼠冠状动脉结扎法制备急性心肌梗死(AMI)模型，在心电图评价的基础上将造模成功的动物随机分为模型组、益气活血组(给予益气活血药治疗)、培哚普利组(给予培哚普利治疗)和通心络组(给予通心络治疗)，以假手术组为对照，共5组，模型组和假手术组给予生理盐水进行干预。选择治疗后第7、28日2个时间点，观察各组大鼠心肌毛细血管密度(MCD)和毛细血管周细胞数量的变化。结果模型组大鼠心肌梗死边缘区MCD较假手术组明显下降、周细胞计数明显增加(P＜0.01)，益气活血组、培哚普利组和通心络组大鼠 MCD 较模型组明显升高，周细胞数量明显下降(P＜0.01)。结论益气活血中药通过降低毛细血管周细胞计数、促进血管新生而达到改善局部心肌血供的目的。
목적관찰심기경사대서모세혈관주세포변화급익기활혈중약적작용，탐토익기활혈중약개선심기혈류관주적작용궤제。방법채용SD대서관상동맥결찰법제비급성심기경사(AMI)모형，재심전도평개적기출상장조모성공적동물수궤분위모형조、익기활혈조(급여익기활혈약치료)、배타보리조(급여배타보리치료)화통심락조(급여통심락치료)，이가수술조위대조，공5조，모형조화가수술조급여생리염수진행간예。선택치료후제7、28일2개시간점，관찰각조대서심기모세혈관밀도(MCD)화모세혈관주세포수량적변화。결과모형조대서심기경사변연구MCD교가수술조명현하강、주세포계수명현증가(P＜0.01)，익기활혈조、배타보리조화통심락조대서 MCD 교모형조명현승고，주세포수량명현하강(P＜0.01)。결론익기활혈중약통과강저모세혈관주세포계수、촉진혈관신생이체도개선국부심기혈공적목적。
Objective To observe the change of capillary pericapillary cells in rats with myocardial infarction and the influence of supplementing qi and activating blood circulation herbs, and explore its mechanism of improving myocardial perfusion. Methods The rat model was established by ligaturing the left anterior descending coronary artery. On the base of ECG evaluation, successfully modeled rats were randomly divided into the model group, group treated with supplementing qi and activating blood circulation Chinese medicine (activating blood and supplementing qi group), group treated with Perindopril (Perindopril group), group treated with Tongxinluo Capsules (Tongxinluo group). The sham-operation group was taken as the control. There were totally 5 groups. The model group and the sham-operation group were treated with normal saline. The changes of myocardial capillary density (MCD) and number of pericapillary cells on the 7th, 28th day after medicinal administration were observed. Results On the 7th and 28th day, the MCD decreased significantly and the number of capillary pericapillary cells increased significantly in the model group compared with the sham-operation group (P<0.01). The MCD increased significantly in the activating blood and supplementing qi group, Perindopril group and Tongxinluo group compared with the model group (P<0.01). The number of pericapillary cells decreased significantly in the activating blood and supplementing qi group, Perindopril group and Tongxinluo group compared with the model group (P<0.01). Conclusion The supplementing qi and activating blood circulation herbs can improve regional myocardial blood supply by decreasing the number of pericytes and promoting regeneration of capillary.