浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
6期
490-492
,共3页
王振%曲丕盛%陶凡%郑汉光
王振%麯丕盛%陶凡%鄭漢光
왕진%곡비성%도범%정한광
家兔%乙醇%腹腔神经丛%毁损%组织学观察
傢兔%乙醇%腹腔神經叢%燬損%組織學觀察
가토%을순%복강신경총%훼손%조직학관찰
rabbits%alcohol%celiac plexus%block%histological observation
目的:以不同浓度乙醇对活体家兔进行腹腔神经丛阻滞毁损,并进行组织病理学观察,为临床治疗提供实验依据。方法成年新西兰大白兔48只,按随机化原则分为手术对照组、50%乙醇组、75%乙醇组和100%乙醇组,各12只。手术对照组给予生理盐水,乙醇各浓度组任选6只共18只在给药1周后活检,其余18只在给药3个月后活检。摘取实验侧腹腔神经节和腹腔神经丛标本,采用Thionine硫堇染色法显示尼氏体,HPIAS-1000病理图文报告系统对尼氏体定量检测平均光密度值。结果1周后,对照组神经节中细胞清晰可见,核大而圆。50%乙醇组,少量神经元核仁消失,少量髓鞘消失。75%乙醇组和100%乙醇组大部分神经元核仁消失,而胞质内尼氏小体变细,髓鞘大部分消失。3个月后,不同浓度乙醇组神经节镜下观结果与1周相似。图像分析显示,与手术对照组比较,乙醇组尼氏体染色平均光密度明显降低(P<0.01);与50%乙醇组比较,75%乙醇组、100%乙醇组1周和3个月尼氏体平均光密度值均明显下降(P<0.05);75%乙醇组与100%乙醇组间差异无统计学意义(P>0.05)。各浓度乙醇组1周与3个月比较,差异无统计学意义(P>0.05)。结论50豫、75豫和100%乙醇对兔腹腔神经丛均有不同程度的变性作用,75豫乙醇是更为适宜的浓度。
目的:以不同濃度乙醇對活體傢兔進行腹腔神經叢阻滯燬損,併進行組織病理學觀察,為臨床治療提供實驗依據。方法成年新西蘭大白兔48隻,按隨機化原則分為手術對照組、50%乙醇組、75%乙醇組和100%乙醇組,各12隻。手術對照組給予生理鹽水,乙醇各濃度組任選6隻共18隻在給藥1週後活檢,其餘18隻在給藥3箇月後活檢。摘取實驗側腹腔神經節和腹腔神經叢標本,採用Thionine硫堇染色法顯示尼氏體,HPIAS-1000病理圖文報告繫統對尼氏體定量檢測平均光密度值。結果1週後,對照組神經節中細胞清晰可見,覈大而圓。50%乙醇組,少量神經元覈仁消失,少量髓鞘消失。75%乙醇組和100%乙醇組大部分神經元覈仁消失,而胞質內尼氏小體變細,髓鞘大部分消失。3箇月後,不同濃度乙醇組神經節鏡下觀結果與1週相似。圖像分析顯示,與手術對照組比較,乙醇組尼氏體染色平均光密度明顯降低(P<0.01);與50%乙醇組比較,75%乙醇組、100%乙醇組1週和3箇月尼氏體平均光密度值均明顯下降(P<0.05);75%乙醇組與100%乙醇組間差異無統計學意義(P>0.05)。各濃度乙醇組1週與3箇月比較,差異無統計學意義(P>0.05)。結論50豫、75豫和100%乙醇對兔腹腔神經叢均有不同程度的變性作用,75豫乙醇是更為適宜的濃度。
목적:이불동농도을순대활체가토진행복강신경총조체훼손,병진행조직병이학관찰,위림상치료제공실험의거。방법성년신서란대백토48지,안수궤화원칙분위수술대조조、50%을순조、75%을순조화100%을순조,각12지。수술대조조급여생리염수,을순각농도조임선6지공18지재급약1주후활검,기여18지재급약3개월후활검。적취실험측복강신경절화복강신경총표본,채용Thionine류근염색법현시니씨체,HPIAS-1000병리도문보고계통대니씨체정량검측평균광밀도치。결과1주후,대조조신경절중세포청석가견,핵대이원。50%을순조,소량신경원핵인소실,소량수초소실。75%을순조화100%을순조대부분신경원핵인소실,이포질내니씨소체변세,수초대부분소실。3개월후,불동농도을순조신경절경하관결과여1주상사。도상분석현시,여수술대조조비교,을순조니씨체염색평균광밀도명현강저(P<0.01);여50%을순조비교,75%을순조、100%을순조1주화3개월니씨체평균광밀도치균명현하강(P<0.05);75%을순조여100%을순조간차이무통계학의의(P>0.05)。각농도을순조1주여3개월비교,차이무통계학의의(P>0.05)。결론50예、75예화100%을순대토복강신경총균유불동정도적변성작용,75예을순시경위괄의적농도。
Objective To observe the relationship between histological changes of neurolytie celiac-plexus block and concentrations of alcohol,in order to provide scientific evidence for clinical application. Methods Forty-eight New Zealand rabbits were randomly divided into 4 groups:control group,50% alcohol group,75% alcohol group,and 100% alcohol group (n=12). Control group was given saline. Eighteen rabbits from alcohol groups (n=6 from each group)were sacrificed one week after administration of alcohol and the rest ones were sacrificed at three month for histological examination. The Nissl body in celiac plexus blocked with alcohol or from the other side without treat-ment were stained with Thionine. HPIAS-1000 system was used to detect OD values of the Nissl body. Results After one week,no abnormality in gangliocytes in tissue sections from control groups was seen;few disappearance of nucleus in neurons was seen in 50% alcohol group;and the majority of nucleus in neurons disappeared in 75% al-cohol group and 100% alcohol group,with smaller Nissl body and demyelination. After three months,the histological changes of alcohol groups were similar to those at one week. The OD values of the Nissl body in alcohol groups were lower than that in control group (P<0.01);the OD values of the Nissl body in 75% alcohol group and 100%alcohol group were lower than that in 50% alcohol group at one week and three months (P<0.05). No significant difference in OD values of the Nissl body was noted between 75% alcohol group and 100% alcohol group (P>0.05). No significant difference was found between one week and three months in each group(P>0.05). Conclusion The histological changes of neurolytie celiac-plexus block was related to the concentration of alcohol. 75% alco-hol may be the suitable one for clinical use.