CAJ | 학술논문

目的：探讨流式细胞术在急性白血病微量残留检测中的应用价值。方法基于初诊急性白血病相关的免疫表型，采用四色流式细胞术、CD45/SSC和CD45/CD19双参数散点图设门，以初发异常的抗体组合检测81例106份完全缓解后急性白血病的微量残留病细胞。结果81例初发急性白血病检测出19种白血病相关的免疫表型，66％患者存在初发抗体表达量的异常。用初发异常抗体组合发现13例存在白血病细胞，为微量残留白血病阳性，8例发生免疫表型变化，占62％。结论根据初诊急性白血病相关的免疫表型，流式细胞术对骨髓中各系造血前体细胞的定性定量检测可用于急性白血病微小残留病变的确诊和早期诊断复发。
목적：탐토류식세포술재급성백혈병미량잔류검측중적응용개치。방법기우초진급성백혈병상관적면역표형，채용사색류식세포술、CD45/SSC화CD45/CD19쌍삼수산점도설문，이초발이상적항체조합검측81례106빈완전완해후급성백혈병적미량잔류병세포。결과81례초발급성백혈병검측출19충백혈병상관적면역표형，66％환자존재초발항체표체량적이상。용초발이상항체조합발현13례존재백혈병세포，위미량잔류백혈병양성，8례발생면역표형변화，점62％。결론근거초진급성백혈병상관적면역표형，류식세포술대골수중각계조혈전체세포적정성정량검측가용우급성백혈병미소잔류병변적학진화조기진단복발。
Objective To evaluate the application value of flow cytometry (FCM ) in screening minimal re-sidual disease (MRD)in acute leukemia(AL) .Methods On the basis the of leukemia-associated immunopheno-types (LAIPs) of 81 with AL ,using incipient abnormal antibodies the MRD of 106 consecutive bone marrow speci-mens from 81 patients who achieved complete remission were detected by 4-color antibody combination ,CD45/SSC and CD45/CD19 gating FCM .Results Ninteen LAIPs were detected in 81 cases with AL .Abnormal expression of incipient antibodies was detected in 81 patients .Thirteen patients were MRD positive and had residual leukemia cells .Eight patients had the modulation of LAIPs.Conclusion According to the LAIPs of AL ,multi-parameter FCM using 4-color antibody panel is appropriate to detect MRD.