世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2013年
8期
862-866,870
,共6页
魏凯%张丹%窦传字%马晓芃%杨玲%吴焕淦%洪珏%朱毅%张翠红%刘婕%吴凌翔%黄燕
魏凱%張丹%竇傳字%馬曉芃%楊玲%吳煥淦%洪玨%硃毅%張翠紅%劉婕%吳凌翔%黃燕
위개%장단%두전자%마효봉%양령%오환감%홍각%주의%장취홍%류첩%오릉상%황연
艾灸%克罗恩病%NF-κB p65%大鼠%效应机制
艾灸%剋囉恩病%NF-κB p65%大鼠%效應機製
애구%극라은병%NF-κB p65%대서%효응궤제
Moxibustion%Crohn disease%NF-κB p65%Rats%Action mechanism
目的：通过观察隔药灸对克罗恩病大鼠结肠NF-κB p65及炎症因子TNF-α、IL-1β表达的影响，探讨艾灸治疗克罗恩病的效应机制。方法：清洁级雄性SD大鼠随机分为4组，分别为正常组、模型组、隔药灸组和西药组，采用三硝基苯磺酸（TN-BS）合乙醇溶液灌肠制备克罗恩病大鼠模型，模型制备成功后，隔药灸组取天枢穴（双）、气海穴进行隔药灸治疗，西药组采用美沙拉秦灌胃治疗。治疗结束后，采用HE染色，光镜下观察各组大鼠结肠组织病理学变化，应用免疫组织化学技术观察各组大鼠结肠NF-κB p65与TNF-α、IL-1β的表达，并进行图像分析。结果：与正常组比较，模型组大鼠结肠组织呈现裂隙状溃疡及炎症表现，部分大鼠结肠在黏膜下层可见肉芽肿形成，结肠NF-κB p65、TNF-α、IL-1β的表达均显著增高（均P＜0.01）；与模型组比较，隔药灸组、西药组大鼠结肠形态结构改善，肠道炎症减轻，结肠NF-κB p65与TNF-α、IL-1β的表达均显著降低（均P＜0.01）；相关性分析结果显示，TNF-α、IL-1β的表达与NF-κB p65的表达均呈正相关性。结论：隔药灸能下调克罗恩病大鼠结肠NF-κB p65、TNF-α、IL-1β的表达；艾灸（隔药灸）可能通过抑制克罗恩病大鼠结肠NF-κB p65的表达，进而减少其下游炎症因子TNF-α、IL-1β的表达，从而减轻肠道炎症，改善结肠组织形态结构，发挥治疗作用。
目的：通過觀察隔藥灸對剋囉恩病大鼠結腸NF-κB p65及炎癥因子TNF-α、IL-1β錶達的影響，探討艾灸治療剋囉恩病的效應機製。方法：清潔級雄性SD大鼠隨機分為4組，分彆為正常組、模型組、隔藥灸組和西藥組，採用三硝基苯磺痠（TN-BS）閤乙醇溶液灌腸製備剋囉恩病大鼠模型，模型製備成功後，隔藥灸組取天樞穴（雙）、氣海穴進行隔藥灸治療，西藥組採用美沙拉秦灌胃治療。治療結束後，採用HE染色，光鏡下觀察各組大鼠結腸組織病理學變化，應用免疫組織化學技術觀察各組大鼠結腸NF-κB p65與TNF-α、IL-1β的錶達，併進行圖像分析。結果：與正常組比較，模型組大鼠結腸組織呈現裂隙狀潰瘍及炎癥錶現，部分大鼠結腸在黏膜下層可見肉芽腫形成，結腸NF-κB p65、TNF-α、IL-1β的錶達均顯著增高（均P＜0.01）；與模型組比較，隔藥灸組、西藥組大鼠結腸形態結構改善，腸道炎癥減輕，結腸NF-κB p65與TNF-α、IL-1β的錶達均顯著降低（均P＜0.01）；相關性分析結果顯示，TNF-α、IL-1β的錶達與NF-κB p65的錶達均呈正相關性。結論：隔藥灸能下調剋囉恩病大鼠結腸NF-κB p65、TNF-α、IL-1β的錶達；艾灸（隔藥灸）可能通過抑製剋囉恩病大鼠結腸NF-κB p65的錶達，進而減少其下遊炎癥因子TNF-α、IL-1β的錶達，從而減輕腸道炎癥，改善結腸組織形態結構，髮揮治療作用。
목적：통과관찰격약구대극라은병대서결장NF-κB p65급염증인자TNF-α、IL-1β표체적영향，탐토애구치료극라은병적효응궤제。방법：청길급웅성SD대서수궤분위4조，분별위정상조、모형조、격약구조화서약조，채용삼초기분광산（TN-BS）합을순용액관장제비극라은병대서모형，모형제비성공후，격약구조취천추혈（쌍）、기해혈진행격약구치료，서약조채용미사랍진관위치료。치료결속후，채용HE염색，광경하관찰각조대서결장조직병이학변화，응용면역조직화학기술관찰각조대서결장NF-κB p65여TNF-α、IL-1β적표체，병진행도상분석。결과：여정상조비교，모형조대서결장조직정현렬극상궤양급염증표현，부분대서결장재점막하층가견육아종형성，결장NF-κB p65、TNF-α、IL-1β적표체균현저증고（균P＜0.01）；여모형조비교，격약구조、서약조대서결장형태결구개선，장도염증감경，결장NF-κB p65여TNF-α、IL-1β적표체균현저강저（균P＜0.01）；상관성분석결과현시，TNF-α、IL-1β적표체여NF-κB p65적표체균정정상관성。결론：격약구능하조극라은병대서결장NF-κB p65、TNF-α、IL-1β적표체；애구（격약구）가능통과억제극라은병대서결장NF-κB p65적표체，진이감소기하유염증인자TNF-α、IL-1β적표체，종이감경장도염증，개선결장조직형태결구，발휘치료작용。
Objective:To explore the action mechanism of moxibustion in treating Crohn′s disease (CD),by observing the effect of herb-partitioned moxibustion on the expressions of NF -κB p65,TNF -α,and IL-1β.Methods:Male Sprague Dawley (SD) rats of clean conventional grade were randomized into four groups ,i.e.a normal control (NC) group,a model control (MC) group,a herb-par-titioned moxibustion (HPM) group,and a Western medication (WM) group.The CD rat models were developed by using the mixture of 2,4,6 trinitrobenzene sulfonic acid (TNBS) and alcohol via enema.When the models were successfully developed ,the HPM group re-ceived HPM intervention at bilateral Tianshu (ST 25) and Qihai (CV 6),and the WM group received Mesalazine via intragastric admin-istration.At the end of treatment,the histopathological variations of rats′colons were observed by adopting HE staining and light micro-scope,and the expressions of NF -κB p65,TNF-α,and IL-1βin rats′colons were determined by using immunohistochemical tech-nique,and the images were all analyzed.Results:Compared to the NC group,the MC group had its rats′colons present with fissured ul-cers and inflammation,and granulomas in submucosa of some colons ,and its expressions of NF -κB p65,TNF -α,and IL-1βin-creased significantly (P<0.01);compared to the MC group,the HPM group and WM group both had rats′colons present with improved structures and reduced intestinal inflammation ,and their expressions of NF -κB p65,TNF-α,and IL-1βdropped significantly (P<0.01);the relevance analyses showed that the expressions of TNF -αand IL-1βboth had a positive correlation with the expression of NF-κB p65.Conclusion:HPM can down-regulate the expressions of NF -κB p65,TNF-α,and IL-1βin CD rats′colons;it′s possible that moxibustion (HPM) produces its therapeutic efficacy by inhibiting the expression of NF -κB p65 in rat′s colon,and subse-quently decreasing the expressions of its downstream inflammatory factors ,i.e.TNF-αand IL-1β,by which,the intestinal inflamma-tion is relieved and the morphology of colon is improved .
