CAJ | 학술논문

将4种氮杂冠醚或吗啉取代的单Schiff碱锰（ III）配合物作为仿水解酶模型催化α-吡啶甲酸对硝基苯酯（ PNPP）水解。考察了单Schiff 碱配体中取代基类型、氮杂冠醚取代的位置对其仿水解酶性能的影响；探讨了Schiff 配合物催化PNPP水解的动力学和机理；提出了配合物催化PNPP水解的动力学模型。结果表明，在25℃条件下随着缓冲溶液 pH值的增大，配合物催化PNPP水解速率提高，氮杂冠醚化单Schiff碱锰（ III）配合物在催化PNPP水解反应中表现出良好的催化活性，Schiff碱配体结构显著影响配合物催化活性。
장4충담잡관미혹마람취대적단Schiff감맹（ III）배합물작위방수해매모형최화α-필정갑산대초기분지（ PNPP）수해。고찰료단Schiff 감배체중취대기류형、담잡관미취대적위치대기방수해매성능적영향；탐토료Schiff 배합물최화PNPP수해적동역학화궤리；제출료배합물최화PNPP수해적동역학모형。결과표명，재25℃조건하수착완충용액 pH치적증대，배합물최화PNPP수해속솔제고，담잡관미화단Schiff감맹（ III）배합물재최화PNPP수해반응중표현출량호적최화활성，Schiff감배체결구현저영향배합물최화활성。
Four mono-Schiff base manganese (III) complexes with either benzo-10-aza-crown ether pendants (MnL12Cl,MnL22Cl, MnL32 Cl) or morpholino pendants( MnL 42 Cl) have been employed as models for hydrolase enzymes by studying the kinetics of their hydrolysis reactions with p-nitrophenyl picolinate ( PNPP) .The effects of the different ligands and the position of the aza-crown in the Schiff base complexes on the kinetics of PNPP hydrolysis were investigated .In addition ,a kinetic mathematical model of PNPP cleavage catalyzed by these complexes was established.The results showed that the rate of the catalytic PNPP hydrolysis increased as the pH of the buffer solution increased .All four complexes exhibited high activity in the catalytic PNPP hydrolysis ,which was in-fluenced by the structures of the different Schiff base ligand .