CAJ | 학술논문

本论文以自行提取的蛋黄卵磷脂为原料，采用薄膜-超声法制备了包含有抗氧化物质Vc的脂质体微囊。以鸡蛋外膜模拟皮肤角质层结构，利用Fenton试剂引入羟基自由基诱导其表面损伤，利用扫描电镜SEM研究了包囊维生素C脂质体对鸡蛋外膜的抗氧化作用效果。以Pyrene为荧光探针，利用荧光探针技术从分子水平上研究了Vc脂质体对羟基自由基的捕获作用。
본논문이자행제취적단황란린지위원료，채용박막-초성법제비료포함유항양화물질Vc적지질체미낭。이계단외막모의피부각질층결구，이용Fenton시제인입간기자유기유도기표면손상，이용소묘전경SEM연구료포낭유생소C지질체대계단외막적항양화작용효과。이Pyrene위형광탐침，이용형광탐침기술종분자수평상연구료Vc지질체대간기자유기적포획작용。
In this dissertation ,egg yolk lecithin was used as the membrane-forming material to prepare antioxidant vitamin C lipo-somes by thin film hydration-ultrasonic method.The outer membrane of eggs was used to bio-mimic skin stratum corneum and dam-aged by introduction of hydroxyl free radicals initiated by Fenton reaction.The protective effect of Vc-liposome on eggshell mem-brane oxidative injury was detected and the morphological changes were observed by scanning electron microscopy (SEM).The fluo -rescence quenching technique was used to investigate the capture roles of vitamine C for OH radicals .