西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2013年
8期
22-24
,共3页
海龙蛤蚧口服液%淫羊藿苷%高效液相法%含量测定
海龍蛤蚧口服液%淫羊藿苷%高效液相法%含量測定
해룡합개구복액%음양곽감%고효액상법%함량측정
HaiLong Gejie oral liquid%icariin%HPLC%content determination
目的：探讨海龙蛤蚧口服液中淫羊藿苷的含量测定方法，为其质量控制提供实验依据。方法：采用高效液相法测定淫羊藿苷的含量，Diamonsil C18柱(250 mm×4.6 mm，5μm)，流动相为乙腈-水(25：75)，流速为1.0 mL/min，最大检测波长为270 nm。结果：淫羊藿苷在0.0232～0.7400μg范围内具有良好的线性关系，回归方程为y＝74988x+22920，R2=1.0，依测定结果，暂定制剂中淫羊藿苷含量为每毫升不低于1.35μg。结论：该方法简便、准确，灵敏度高，重现性好，可作为海龙蛤蚧口服液质量控制的有效方法。
目的：探討海龍蛤蚧口服液中淫羊藿苷的含量測定方法，為其質量控製提供實驗依據。方法：採用高效液相法測定淫羊藿苷的含量，Diamonsil C18柱(250 mm×4.6 mm，5μm)，流動相為乙腈-水(25：75)，流速為1.0 mL/min，最大檢測波長為270 nm。結果：淫羊藿苷在0.0232～0.7400μg範圍內具有良好的線性關繫，迴歸方程為y＝74988x+22920，R2=1.0，依測定結果，暫定製劑中淫羊藿苷含量為每毫升不低于1.35μg。結論：該方法簡便、準確，靈敏度高，重現性好，可作為海龍蛤蚧口服液質量控製的有效方法。
목적：탐토해룡합개구복액중음양곽감적함량측정방법，위기질량공제제공실험의거。방법：채용고효액상법측정음양곽감적함량，Diamonsil C18주(250 mm×4.6 mm，5μm)，류동상위을정-수(25：75)，류속위1.0 mL/min，최대검측파장위270 nm。결과：음양곽감재0.0232～0.7400μg범위내구유량호적선성관계，회귀방정위y＝74988x+22920，R2=1.0，의측정결과，잠정제제중음양곽감함량위매호승불저우1.35μg。결론：해방법간편、준학，령민도고，중현성호，가작위해룡합개구복액질량공제적유효방법。
Objective: To provide experimental evidence for quality control of HaiLong Gejie oral liquid through discussing the content determination method of icariin. Method:High performance liquid chromatography (HPLC) was used for the content determination of icariin, the column was Diamonsil C18 (250 mm×4.6 mm, 5μm), the mobile phase was acetonitrile-water (25:75), the flow rate was 1.0 mL/min, the detection wavelength was 270nm. Result:Icariin showed the better linear relationship in the range of 0.023 2~0.74 00 μg, and the regression equation was y=74988x+22920, R2=1.0. Based on the test results, the contents of icariin in the tentative preparations were not less than 1.35μg/mL. Conclusion:The method, simple, accurate, sensitive and reproducible, is effective for quality control of HaiLong Gejie oral liquid.
