中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
9期
653-658
,共6页
Th17细胞%Treg细胞%结核性胸膜炎%流式细胞术%细胞因子
Th17細胞%Treg細胞%結覈性胸膜炎%流式細胞術%細胞因子
Th17세포%Treg세포%결핵성흉막염%류식세포술%세포인자
Th17 cell%Treg cell%Tuberculous pleurisy%Flow cytometry%Cytokine
目的检测健康人和结核性胸膜炎患者外周血Th17细胞和调节性T细胞( Treg细胞)( CD4+CD25+Foxp3+)在CD4+T细胞中的表达率以及IL-17、IL-23、IL-6、TGF-β血清水平和患者胸水中的IL-17、IL-23、IL-6、TGF-β水平,研究Th17细胞和调节性T细胞以及IL-17、IL-23、IL-6、TGF-β在结核性胸膜炎发病机制中的作用。方法使用流式细胞术检测患者以及健康对照人群外周血Th17细胞和调节性T细胞表达率,ELSIA方法定量检测血清以及胸水中IL-17、IL-23、IL-6、TGF-β水平,使用SPSS17.0统计学软件,分析健康人和结核性胸膜炎患者上述指标之间的差异以及各指标间的相关性。结果结核性胸膜炎患者外周血Th17细胞表达率(1.02%±0.20%)明显高于健康人外周血Th17细胞表达率(0.89%±0.13%,P=0.002<0.05);结核性胸膜炎患者外周血调节性T细胞表达率(4.64%±0.77%)明显低于健康人外周血调节性T细胞表达率(5.10%±0.90%,P=0.000<0.05);结核性胸膜炎患者Th17/Treg细胞的比率(0.25±0.07)明显高于健康人(0.17±0.05,P=0.000<0.05);结核性胸膜炎患者外周血IL-17(17.49 ng/L±3.94 ng/L)和IL-23(90.42 ng/L±23.06 ng/L)水平和胸水中IL-17(26.13 ng/L±5.98 ng/L)和IL-23(122.26 ng/L±31.71 ng/L)水平显著高于对照组外周血IL-17(14.45 ng/L±3.81 ng/L)和IL-23(77.55 ng/L±20.26 ng/L)的水平,P值分别为0.022、0.039、0.000、0.000;患者胸水中IL-17和IL-23浓度也显著高于本人血液中的IL-17和IL-23浓度,P值为0.000和0.000;患者胸水中IL-6的浓度(5.31 ng/L±0.74 ng/L)显著高于患者血液中IL-6的浓度(4.54 ng/L±1.02 ng/L)和对照组血液中IL-6的浓度(4.26 ng/L±0.91 ng/L),P值分别为0.003和0.000,患者血液与对照组血液中IL-6的浓度没有显著差别(P=0.274);对照组外周血液TGF-β浓度(3.95 ng/L±0.79 ng/L)显著高于患者外周血液TGF-β浓度(3.32 ng/L±0.80 ng/L)及胸水中TGF-β浓度(3.12±0.77),P值分别为0.005和0.000,患者血液及胸水中TGF-β水平之间没有显著差别(P=0.365);结核性胸膜炎患者外周血Th17细胞的表达率与其Treg细胞在外周血的表达率呈显著负相关(r=-0.684, P=0.000<0.05),结核性胸膜炎患者外周血Th17细胞的表达率与其外周血中的IL-17、IL-23、IL-6水平呈明显的正相关(r=0.479,0.441,0.326,P=0.013,0.015,0.017);患者血液中TGF-β水平与Treg细胞在外周血的表达率呈明显的正相关(r=0.297,P=0.024),与Th17细胞表达率没有明显相关性(r=0.091,P=0.659)。结论 Th17和Treg细胞可能参与了结核性胸膜炎的免疫病理机制,有关细胞因子的变化可能参与了Th17和Treg细胞变化的调控以及炎症反应, Th17和Treg细胞以及有关细胞因子的变化可能是结核性胸膜炎重要的免疫病理机制。
目的檢測健康人和結覈性胸膜炎患者外週血Th17細胞和調節性T細胞( Treg細胞)( CD4+CD25+Foxp3+)在CD4+T細胞中的錶達率以及IL-17、IL-23、IL-6、TGF-β血清水平和患者胸水中的IL-17、IL-23、IL-6、TGF-β水平,研究Th17細胞和調節性T細胞以及IL-17、IL-23、IL-6、TGF-β在結覈性胸膜炎髮病機製中的作用。方法使用流式細胞術檢測患者以及健康對照人群外週血Th17細胞和調節性T細胞錶達率,ELSIA方法定量檢測血清以及胸水中IL-17、IL-23、IL-6、TGF-β水平,使用SPSS17.0統計學軟件,分析健康人和結覈性胸膜炎患者上述指標之間的差異以及各指標間的相關性。結果結覈性胸膜炎患者外週血Th17細胞錶達率(1.02%±0.20%)明顯高于健康人外週血Th17細胞錶達率(0.89%±0.13%,P=0.002<0.05);結覈性胸膜炎患者外週血調節性T細胞錶達率(4.64%±0.77%)明顯低于健康人外週血調節性T細胞錶達率(5.10%±0.90%,P=0.000<0.05);結覈性胸膜炎患者Th17/Treg細胞的比率(0.25±0.07)明顯高于健康人(0.17±0.05,P=0.000<0.05);結覈性胸膜炎患者外週血IL-17(17.49 ng/L±3.94 ng/L)和IL-23(90.42 ng/L±23.06 ng/L)水平和胸水中IL-17(26.13 ng/L±5.98 ng/L)和IL-23(122.26 ng/L±31.71 ng/L)水平顯著高于對照組外週血IL-17(14.45 ng/L±3.81 ng/L)和IL-23(77.55 ng/L±20.26 ng/L)的水平,P值分彆為0.022、0.039、0.000、0.000;患者胸水中IL-17和IL-23濃度也顯著高于本人血液中的IL-17和IL-23濃度,P值為0.000和0.000;患者胸水中IL-6的濃度(5.31 ng/L±0.74 ng/L)顯著高于患者血液中IL-6的濃度(4.54 ng/L±1.02 ng/L)和對照組血液中IL-6的濃度(4.26 ng/L±0.91 ng/L),P值分彆為0.003和0.000,患者血液與對照組血液中IL-6的濃度沒有顯著差彆(P=0.274);對照組外週血液TGF-β濃度(3.95 ng/L±0.79 ng/L)顯著高于患者外週血液TGF-β濃度(3.32 ng/L±0.80 ng/L)及胸水中TGF-β濃度(3.12±0.77),P值分彆為0.005和0.000,患者血液及胸水中TGF-β水平之間沒有顯著差彆(P=0.365);結覈性胸膜炎患者外週血Th17細胞的錶達率與其Treg細胞在外週血的錶達率呈顯著負相關(r=-0.684, P=0.000<0.05),結覈性胸膜炎患者外週血Th17細胞的錶達率與其外週血中的IL-17、IL-23、IL-6水平呈明顯的正相關(r=0.479,0.441,0.326,P=0.013,0.015,0.017);患者血液中TGF-β水平與Treg細胞在外週血的錶達率呈明顯的正相關(r=0.297,P=0.024),與Th17細胞錶達率沒有明顯相關性(r=0.091,P=0.659)。結論 Th17和Treg細胞可能參與瞭結覈性胸膜炎的免疫病理機製,有關細胞因子的變化可能參與瞭Th17和Treg細胞變化的調控以及炎癥反應, Th17和Treg細胞以及有關細胞因子的變化可能是結覈性胸膜炎重要的免疫病理機製。
목적검측건강인화결핵성흉막염환자외주혈Th17세포화조절성T세포( Treg세포)( CD4+CD25+Foxp3+)재CD4+T세포중적표체솔이급IL-17、IL-23、IL-6、TGF-β혈청수평화환자흉수중적IL-17、IL-23、IL-6、TGF-β수평,연구Th17세포화조절성T세포이급IL-17、IL-23、IL-6、TGF-β재결핵성흉막염발병궤제중적작용。방법사용류식세포술검측환자이급건강대조인군외주혈Th17세포화조절성T세포표체솔,ELSIA방법정량검측혈청이급흉수중IL-17、IL-23、IL-6、TGF-β수평,사용SPSS17.0통계학연건,분석건강인화결핵성흉막염환자상술지표지간적차이이급각지표간적상관성。결과결핵성흉막염환자외주혈Th17세포표체솔(1.02%±0.20%)명현고우건강인외주혈Th17세포표체솔(0.89%±0.13%,P=0.002<0.05);결핵성흉막염환자외주혈조절성T세포표체솔(4.64%±0.77%)명현저우건강인외주혈조절성T세포표체솔(5.10%±0.90%,P=0.000<0.05);결핵성흉막염환자Th17/Treg세포적비솔(0.25±0.07)명현고우건강인(0.17±0.05,P=0.000<0.05);결핵성흉막염환자외주혈IL-17(17.49 ng/L±3.94 ng/L)화IL-23(90.42 ng/L±23.06 ng/L)수평화흉수중IL-17(26.13 ng/L±5.98 ng/L)화IL-23(122.26 ng/L±31.71 ng/L)수평현저고우대조조외주혈IL-17(14.45 ng/L±3.81 ng/L)화IL-23(77.55 ng/L±20.26 ng/L)적수평,P치분별위0.022、0.039、0.000、0.000;환자흉수중IL-17화IL-23농도야현저고우본인혈액중적IL-17화IL-23농도,P치위0.000화0.000;환자흉수중IL-6적농도(5.31 ng/L±0.74 ng/L)현저고우환자혈액중IL-6적농도(4.54 ng/L±1.02 ng/L)화대조조혈액중IL-6적농도(4.26 ng/L±0.91 ng/L),P치분별위0.003화0.000,환자혈액여대조조혈액중IL-6적농도몰유현저차별(P=0.274);대조조외주혈액TGF-β농도(3.95 ng/L±0.79 ng/L)현저고우환자외주혈액TGF-β농도(3.32 ng/L±0.80 ng/L)급흉수중TGF-β농도(3.12±0.77),P치분별위0.005화0.000,환자혈액급흉수중TGF-β수평지간몰유현저차별(P=0.365);결핵성흉막염환자외주혈Th17세포적표체솔여기Treg세포재외주혈적표체솔정현저부상관(r=-0.684, P=0.000<0.05),결핵성흉막염환자외주혈Th17세포적표체솔여기외주혈중적IL-17、IL-23、IL-6수평정명현적정상관(r=0.479,0.441,0.326,P=0.013,0.015,0.017);환자혈액중TGF-β수평여Treg세포재외주혈적표체솔정명현적정상관(r=0.297,P=0.024),여Th17세포표체솔몰유명현상관성(r=0.091,P=0.659)。결론 Th17화Treg세포가능삼여료결핵성흉막염적면역병리궤제,유관세포인자적변화가능삼여료Th17화Treg세포변화적조공이급염증반응, Th17화Treg세포이급유관세포인자적변화가능시결핵성흉막염중요적면역병리궤제。
Objective To investigate the significance of Th 17 cells, Treg cells and Th17/Treg cell-associated cytokines in the development of tuberculous pleurisy through detecting the expressions of Th 17 cells and Treg cells (CD4+CD25+Foxp3+) in CD4+T cells, analyzing concentrations of IL-17, IL-23, IL-6 and TGF-βin serum of patients with tuberculous pleurisy and healthy controls and measuring levels of IL -17, IL-23, IL-6 and TGF-βin hydrothorax of patients with tuberculous pleurisy .Methods Flow cytometry was used to detect expressions of Th 17 and Treg cells in peripheral blood of patients with tuberculous pleurisy and healthy controls.ELISA method was performed for quantitative detection of concentrations of IL -17, IL-23, IL-6 and TGF-βin serum and hydrothorax .Differences and correlations between the above measured data were analyzed by the statistical software SPSS 17.0.Results Compared with the healthy control group , the expressions of Th17 cells in peripheral blood of the patients were significantly increased (1.02%±0.20%vs.0.89%±0.13%, P=0.002<0.05), while the expressions of Treg cells were significantly decreased (4.64%±0.77%vs.5.10%±0.90%, P=0.000<0.05).Correspondingly, the ratio of Th17/Treg cells (0.25±0.07) in the patients were significantly higher than that in the healthy controls (0.17±0.05, P=0.000<0.05).Concentrations of IL-17, IL-23 and IL-6 in peripheral blood and in hydrothorax of the pa-tients were (17.49±3.94) ng/L, (90.42±23.06) ng/L, (4.54±1.02) ng/L and (26.13±5.98) ng/L, (122.26±31.71) ng/L, (5.31±0.74) ng/L respectively, which were remarkably higher than the levels of IL-17 (14.45±3.81) ng/L, IL-23 (77.55±20.26) ng/L and IL-6 (4.26±0.91) ng/L in control group. In tuberculous pleurisy group , concentrations of IL-17, IL-23 and IL-6 in hydrothorax were significantly higher than those in peripheral blood with P values of 0.000, 0.000 and 0.003.There was no difference be-tween IL-6 levels in peripheral blood from patients and IL-6 levels in peripheral blood from the healthy con-trols, P=0.274.In comparison with the control group , TGF-βlevels in peripheral blood and in hydrothorax of the patients were significantly decreased (3.95 ng/L±0.79 ng/L, 3.12 ng/L±0.77 ng/L vs.3.32 ng/L ±0.80 ng/L) .In tuberculous pleurisy patients , the expression of Th17 cells in peripheral blood was nega-tively correlated with Treg cells in peripheral blood (r=-0.684, P=0.000<0.05), but was positively relat-ed to the levels of IL-17, IL-23 and IL-6 (r=0.479, 0.441, 0.326, P=0.013, 0.015, 0.017).TGF-βlevel had significantly positive correlations with Treg cells in the peripheral blood of the patients (r=0.297, P=0.024), but no significant correlation with Th17 cells was found (r=0.091, P=0.659).Conclusion Th17/Treg cell-associated cytokines might regulate the expressions of Th 17 and Treg cells and inflammatory reaction.Changes of Th17 cells, Treg cells and related cytokines might be important immunopathological mechanisms for tuberculous pleurisy .