CAJ | 학술논문

[目的]应用双向电泳及质谱技术研究白藜芦醇(Resveratrol，Res)对宫颈癌HeLa细胞蛋白质组的影响。[方法]经磺基罗丹明B(sulforhodamine B ,SRB)法筛选对宫颈癌HeLa细胞有效的白藜芦醇浓度，流式细胞仪检测有效白藜芦醇浓度对HeLa细胞周期和凋亡的影响。HeLa细胞经白藜芦醇处理后，提取细胞总蛋白，应用双向电泳技术建立蛋白质组图谱，筛选白藜芦醇处理前后差异在2倍以上的蛋白质作为差异蛋白，进行质谱鉴定和生物信息学分析。[结果]50μmol/L以上浓度白藜芦醇24小时对HeLa细胞的活性产生影响，将细胞阻滞在S期，诱导HeLa细胞的早期凋亡，并呈剂量依赖性。通过差异蛋白质组分析筛选出12个差异蛋白质，包括原肌球蛋白、细胞核糖核蛋白C、锌指蛋白、钙连接蛋白、微管蛋白β、热休克蛋白70、α烯醇化酶、丙酮酸激酶、雌激素受体β、氨基甲酰磷酸合成酶，HeLa细胞经白藜芦醇处理后这些蛋白表达量均增加。[结论]白藜芦醇可能通过参与调节细胞的转移分化、凋亡、能量代谢等过程而起到抗肿瘤作用。
[목적]응용쌍향전영급질보기술연구백려호순(Resveratrol，Res)대궁경암HeLa세포단백질조적영향。[방법]경광기라단명B(sulforhodamine B ,SRB)법사선대궁경암HeLa세포유효적백려호순농도，류식세포의검측유효백려호순농도대HeLa세포주기화조망적영향。HeLa세포경백려호순처리후，제취세포총단백，응용쌍향전영기술건립단백질조도보，사선백려호순처리전후차이재2배이상적단백질작위차이단백，진행질보감정화생물신식학분석。[결과]50μmol/L이상농도백려호순24소시대HeLa세포적활성산생영향，장세포조체재S기，유도HeLa세포적조기조망，병정제량의뢰성。통과차이단백질조분석사선출12개차이단백질，포괄원기구단백、세포핵당핵단백C、자지단백、개련접단백、미관단백β、열휴극단백70、α희순화매、병동산격매、자격소수체β、안기갑선린산합성매，HeLa세포경백려호순처리후저사단백표체량균증가。[결론]백려호순가능통과삼여조절세포적전이분화、조망、능량대사등과정이기도항종류작용。
[Objective] To explore the effects of resveratrol on the proteome of human cervical carinoma HeLa cellline.[Methods] Proliferation activity was tested by sulforhodamine B(SRB) method for resveratrol effect concentration in HeLa cells, cellcycle and apoptosis of HeLa cells treated with different resveratrol concentrations were analysed by flow cytometry. The total proteins were extracted after resveratrol-treated HeLa cells for 24h and analyzed by 2-dimentional gel electrophoresis, then MALDI-TOF-MS was used to provide mass spectral data for unique proteins which were increased or decreased more than 2 folders compared with control group. [Results] Resveratrol could affect Hela cellproliferation activity over 50μmol/L concentration and pre-sented concentration-dependent, arrested cellcycle at S phase and conduced early apoptosis of HeLa cells. 2-DE images results showed that 12 proteins had been identified, such as tropomyosin, nuclear ribonucleoprotein C, ZNF, calcium binding protein, tubulin beta, HSP 70, alpha enolase, pyruvate ki-nase, estrogen receptor beta, carbamoylphosphate synthetase and so on after treatment with 50μmol/L resveratrol for 24 hours. [Conclusion] Resveratrol could resist HeLa celland its mechanism may be regulating cytoplasmic movement, apoptosis and energy metabolism.