中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2013年
9期
68-71
,共4页
刘春菊%孙成友%赵永刚%吴晓东%刘文华%邹艳丽%任炜杰%王志亮
劉春菊%孫成友%趙永剛%吳曉東%劉文華%鄒豔麗%任煒傑%王誌亮
류춘국%손성우%조영강%오효동%류문화%추염려%임위걸%왕지량
猪%细环病毒Ⅰ型%PCR%琼脂糖凝胶电泳%分子流行病学调查
豬%細環病毒Ⅰ型%PCR%瓊脂糖凝膠電泳%分子流行病學調查
저%세배병독Ⅰ형%PCR%경지당응효전영%분자류행병학조사
Swine%Torque teno virus type%Polymerase chain reaction%Agarose gel electrophoresis%Molecular epidemiological investigation
为了解猪细环病毒Ⅰ型(TTV1)的流行情况,对莱西、城阳、胶州、莱阳四家规模化养猪场的59份血清进行了TTV1分子流行病学调查,从血清中提取病毒DNA,然后通过TTV1引物进行PCR检测样品的感染情况。检测结果表明,所检测的4家养猪场均检测到TTV1感染。其中,莱西某猪场的感染率最高,为55.2%;莱阳、城阳、胶州猪场的感染率分别为30%、10%、20%。目前TTV1不能造成直接的致病作用,但是在我国很容易与其他病原发生混合感染,导致协同致病,因此了解TTV1在猪场中的存在情况具有重要意义。
為瞭解豬細環病毒Ⅰ型(TTV1)的流行情況,對萊西、城暘、膠州、萊暘四傢規模化養豬場的59份血清進行瞭TTV1分子流行病學調查,從血清中提取病毒DNA,然後通過TTV1引物進行PCR檢測樣品的感染情況。檢測結果錶明,所檢測的4傢養豬場均檢測到TTV1感染。其中,萊西某豬場的感染率最高,為55.2%;萊暘、城暘、膠州豬場的感染率分彆為30%、10%、20%。目前TTV1不能造成直接的緻病作用,但是在我國很容易與其他病原髮生混閤感染,導緻協同緻病,因此瞭解TTV1在豬場中的存在情況具有重要意義。
위료해저세배병독Ⅰ형(TTV1)적류행정황,대래서、성양、효주、래양사가규모화양저장적59빈혈청진행료TTV1분자류행병학조사,종혈청중제취병독DNA,연후통과TTV1인물진행PCR검측양품적감염정황。검측결과표명,소검측적4가양저장균검측도TTV1감염。기중,래서모저장적감염솔최고,위55.2%;래양、성양、효주저장적감염솔분별위30%、10%、20%。목전TTV1불능조성직접적치병작용,단시재아국흔용역여기타병원발생혼합감염,도치협동치병,인차료해TTV1재저장중적존재정황구유중요의의。
In order to investigate the prevalence of porcine torque teno virus type Ⅰ(TTV1),59 serum samples from four large-scale pig farms in Laixi,Laiyang,Jiaozhou and Chengyang were collected for molecular epidemiological investigation of TTV1. DNA was extracted from serum by a High Pure Viral Nucleic Acid Kit,and PCR was conducted using the specific primers of TTVI. Results showed that the infection of TTV1 was detected in all the four selected pig farms. Among the four farms,the highest infection rate was 55.2%in Laixi. The infection rates of farms in Laiyang,Jiaozhou,and Chengyang were 30%,10%,and 20%respectively,indicating that TTV1 existed widely in pig farms. TTV1 usually is not a pathogenic agent,but it can enhance the pathogenicity of the other pathogens. Therefore,it is important to be aware of the infection of TTV1 in pig population.