中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2013年
9期
64-67
,共4页
熊炜%蒋静%张强%盘宝进%李健%魏晓锋%黄忠荣%胡建华
熊煒%蔣靜%張彊%盤寶進%李健%魏曉鋒%黃忠榮%鬍建華
웅위%장정%장강%반보진%리건%위효봉%황충영%호건화
猴%猴嗜T淋巴细胞病毒l型%RT-PCR%Real-time RT-PCR%Taqman探针
猴%猴嗜T淋巴細胞病毒l型%RT-PCR%Real-time RT-PCR%Taqman探針
후%후기T림파세포병독l형%RT-PCR%Real-time RT-PCR%Taqman탐침
monkey%simian T-cell lymphotropic virus type 1%RT-PCR%real-time RT-PCR%Taqman probe
猴嗜T淋巴细胞病毒l型（Simian T-cell lymphotropic virus type 1，STLV-1）是无特定病原体（SPF）猴必须排除的病毒之一，其潜伏期较长，主要侵害猴的免疫系统。为强化口岸对进出境野生及实验用灵长类动物STLV-1感染情况的监测和流行病学调查，本研究建立了RT-PCR和Real-time RT-PCR检测STLV-1的方法，并对方法的特异性、敏感性和稳定性进行了确认。
猴嗜T淋巴細胞病毒l型（Simian T-cell lymphotropic virus type 1，STLV-1）是無特定病原體（SPF）猴必鬚排除的病毒之一，其潛伏期較長，主要侵害猴的免疫繫統。為彊化口岸對進齣境野生及實驗用靈長類動物STLV-1感染情況的鑑測和流行病學調查，本研究建立瞭RT-PCR和Real-time RT-PCR檢測STLV-1的方法，併對方法的特異性、敏感性和穩定性進行瞭確認。
후기T림파세포병독l형（Simian T-cell lymphotropic virus type 1，STLV-1）시무특정병원체（SPF）후필수배제적병독지일，기잠복기교장，주요침해후적면역계통。위강화구안대진출경야생급실험용령장류동물STLV-1감염정황적감측화류행병학조사，본연구건립료RT-PCR화Real-time RT-PCR검측STLV-1적방법，병대방법적특이성、민감성화은정성진행료학인。
Simian T-cell lymphotropic virus type 1 (STLV-1) is one of viruses required to be free in specefic pathogen free (SPF) monkey.The virus has a long infective period and damages the immune system of monkey. In order to survey and investigate the epidemic characters of STLV-1 transmission among wild and experimental monkeys, methods of RT-PCR and Real-time RT-PCR using TaqMan probe to detect STLV-1 were established and proved to be specific with high sensitivity and stability.
