CAJ | 학술논문

采用柠檬酸三钠还原法制备胶体金颗粒，并将其标记SPA，将金标SPA包被至玻璃纤维膜上制备金标垫，将纯化的Cap蛋白、兔抗SPA抗体分别包被至硝酸纤维素膜的检测线与质控线上，建立了一种检测猪圆环病毒2型抗体的胶体金免疫层析方法。检测结果显示，组装的检测卡只与圆环病毒阳性血清发生特异性反应，检测的灵敏度可以稀释到100倍。用此检测卡与商品化ELISA试剂盒对86份临床血清进行平行检测，两者的检测阳性率分别为65.12%和70.93%。该方法检测速度快，15min内可出结果，特异性强，操作简便，可广泛应用于基层。
채용저몽산삼납환원법제비효체금과립，병장기표기SPA，장금표SPA포피지파리섬유막상제비금표점，장순화적Cap단백、토항SPA항체분별포피지초산섬유소막적검측선여질공선상，건립료일충검측저원배병독2형항체적효체금면역층석방법。검측결과현시，조장적검측잡지여원배병독양성혈청발생특이성반응，검측적령민도가이희석도100배。용차검측잡여상품화ELISA시제합대86빈림상혈청진행평행검측，량자적검측양성솔분별위65.12%화70.93%。해방법검측속도쾌，15min내가출결과，특이성강，조작간편，가엄범응용우기층。
Staphylococcal protein A(SPA)labeled with colloidal gold particles prepared by trisodium citrate reduction and the labeled SPA was coated onto glass fibrous membrane to prepare gold labeled pad. The purified Cap protein of porcine circovirus 2 and rabbit anti-SPA antibody were coated respectively to the test line and quality control line on nitrate cellulose membrane,so as to establish a colloidal gold immuno-chromatography for detection of antibodies against porcine circovirus-2. The results indicated that the assembled test card reacted exclusively with PCV-2 positive sera and the test sensitivity was up-to 100 fold dilutions. 86 clinical samples were tested both with the test card and the commercial ELISAkid,resulting in positive rates of 65.12%and 70.93%respectively. The method was rapid, specific and simple in operation,and could be extended at grass-roots.