中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2013年
9期
56-58
,共3页
赵祥平%王建华%董志珍%王玉玲%王乃福%张永年%刘亚峰%刘红梅
趙祥平%王建華%董誌珍%王玉玲%王迺福%張永年%劉亞峰%劉紅梅
조상평%왕건화%동지진%왕옥령%왕내복%장영년%류아봉%류홍매
牛白血病%ELISA%AGID%PCR%比较试验
牛白血病%ELISA%AGID%PCR%比較試驗
우백혈병%ELISA%AGID%PCR%비교시험
Bovine leukemia%ELISA%AGID%PCR%Comparative test
为比较ELISA、AGID和PCR方法检测牛白血病的检出率和符合率，本试验采用这三种方法对隔离期间总数为3998头的乌拉圭进口奶牛进行了牛白血病检测。试验结果表明，对奶牛的牛白血病检测以ELISA方法检出率最高，检出牛白血病抗体阳性牛23头，检出率为0.58%（23/3998）；AGID方法检出牛白血病抗体阳性牛18头，检出率为0.45%（18/3998），与ELISA方法的符合率为78%；而PCR方法检出牛白血病前病毒核酸阳性牛12头，检出率为0.30%（12/3998），与ELISA方法和AGID方法的符合率分别为43%和55%。
為比較ELISA、AGID和PCR方法檢測牛白血病的檢齣率和符閤率，本試驗採用這三種方法對隔離期間總數為3998頭的烏拉圭進口奶牛進行瞭牛白血病檢測。試驗結果錶明，對奶牛的牛白血病檢測以ELISA方法檢齣率最高，檢齣牛白血病抗體暘性牛23頭，檢齣率為0.58%（23/3998）；AGID方法檢齣牛白血病抗體暘性牛18頭，檢齣率為0.45%（18/3998），與ELISA方法的符閤率為78%；而PCR方法檢齣牛白血病前病毒覈痠暘性牛12頭，檢齣率為0.30%（12/3998），與ELISA方法和AGID方法的符閤率分彆為43%和55%。
위비교ELISA、AGID화PCR방법검측우백혈병적검출솔화부합솔，본시험채용저삼충방법대격리기간총수위3998두적오랍규진구내우진행료우백혈병검측。시험결과표명，대내우적우백혈병검측이ELISA방법검출솔최고，검출우백혈병항체양성우23두，검출솔위0.58%（23/3998）；AGID방법검출우백혈병항체양성우18두，검출솔위0.45%（18/3998），여ELISA방법적부합솔위78%；이PCR방법검출우백혈병전병독핵산양성우12두，검출솔위0.30%（12/3998），여ELISA방법화AGID방법적부합솔분별위43%화55%。
To compare the detection rate and coincidence rate of ELISA、AGID and PCR for the detection of bovine leukemia,3998 cows imported from Uruguay were detected by three method during the quarantine period. The test results showed that 23 were positive for bovine leukemia antibody by ELISA with the highest detection rate of 0.58%.18 were positive for bovine leukemia antibody by AGID with the detection rate of 0.45%. 12 were positive for bovine leukemia provirus DNA by PCR with the detection rate of 0.30. The conformation rate of AGID to ELISA is 78%,and the conformation rate of PCR to ELISA and AGID are 43%and 55%respectively.
