CAJ | 학술논문

目的探讨比较单纯性肥胖儿童及正常儿童血清瘦素、生长激素（growth hormone GH）生物、免疫活性及瘦素水平，为临床进一步明确儿童单纯性肥胖的病因提供新的方向。方法随机选取单纯性肥胖儿童68例（单纯性肥胖组）和正常儿童72例（正常对照组），均空腹抽取静脉血离心，包被试管放射免疫法定测定瘦素水平， BAF /GM细胞株法测定血清GH生物活性，免疫放射分析法（IRMA）测定血清人GH的免疫活性，并评估两组的B/I比值。结果单纯性肥胖组血清样本G H 生物活性和免疫活性的平均值分别为（1．26±0．50）、（2．24±0．72），正常对照组G H生物活性和免疫活性分别为（2．18±0．77）、（2．07±0．66），两组生长激素生物活性与免疫活性比较差异无统计学意义（ P ＞0．05）。正常对照组血清样本的B/I比值平均为（1．02±0．21），单纯性肥胖组为（0．53±0．13），两组的B/I比值比较差异有统计学意义（P ＜0．05）。单纯性肥胖组血清瘦素含量为（23．58±4．35），正常对照组血清瘦素含量为（6．95±3．61）。单纯性肥胖组血清瘦素水平高于正常对照组（ P ＜0．05）。结论单纯性肥胖儿童瘦素水平较正常儿童增高，可能存在瘦素抵抗现象。单纯性肥胖儿童与正常儿童比较生长激素生物活性明显减低。
목적탐토비교단순성비반인동급정상인동혈청수소、생장격소（growth hormone GH）생물、면역활성급수소수평，위림상진일보명학인동단순성비반적병인제공신적방향。방법수궤선취단순성비반인동68례（단순성비반조）화정상인동72례（정상대조조），균공복추취정맥혈리심，포피시관방사면역법정측정수소수평， BAF /GM세포주법측정혈청GH생물활성，면역방사분석법（IRMA）측정혈청인GH적면역활성，병평고량조적B/I비치。결과단순성비반조혈청양본G H 생물활성화면역활성적평균치분별위（1．26±0．50）、（2．24±0．72），정상대조조G H생물활성화면역활성분별위（2．18±0．77）、（2．07±0．66），량조생장격소생물활성여면역활성비교차이무통계학의의（ P ＞0．05）。정상대조조혈청양본적B/I비치평균위（1．02±0．21），단순성비반조위（0．53±0．13），량조적B/I비치비교차이유통계학의의（P ＜0．05）。단순성비반조혈청수소함량위（23．58±4．35），정상대조조혈청수소함량위（6．95±3．61）。단순성비반조혈청수소수평고우정상대조조（ P ＜0．05）。결론단순성비반인동수소수평교정상인동증고，가능존재수소저항현상。단순성비반인동여정상인동비교생장격소생물활성명현감저。
Objective To investigate the comparison of normal children with simple obesity children and se-rum leptin ,GH (growth hormone GH) biological and immunological activity ,clinical further clarify the e-tiology of obesity of children provided a new direction .Methods 68 cases were randomly selected child is obese group and 72 cases of normal control group children were fasting venous blood was centrifuged ,coa-ted tube radioimmunoassay statutory determination of leptin ,BAF/GM cell line method for measuring ser-um GH biological activity ,immune Assay method (IRMA) human serum GH immune activity and to eval-uate the two groups B/I ratios .Results Obesity GH serum samples of biological activity and immunologi-cal activity average values were (1 .26 ± 0 .50) ,(2 .24 ± 0 .72) .Normal control group GH biological activi-ty and immunological activity were (2 .18 ± 0 .77) ,(2 .07 ± 0 .66) .Two immunoreactive growth hormone bioactivity compared with no significant difference (P>0 .05) .Serum samples from normal control group , the average B/I ratio was (1 .02 ± 0 .21) ,obese group was (0 .53 ± 0 .13) .Two groups of B/I ratio was statistically significant difference (P<0 .05) .Obesity serum leptin concentration was (23 .58 ± 4 .35) ,the normal control group ,serum leptin concentration was (6 .95 ± 3 .61) .Serum leptin levels in obese group than the control group (P <0 .05) .Conclusion Obese children compared with normal children increased levels of leptin ,leptin resistance phenomenon may exist .Obese children and normal children grow th hor-mone and immune biological active substance significantly different .