新疆医科大学学报
新疆醫科大學學報
신강의과대학학보
JOURNAL OF XINJIANG MEDICAL UNIVERSITY
2013年
9期
1253-1257
,共5页
马琪%马小娟%姚雪萍%于文燕%孙湛
馬琪%馬小娟%姚雪萍%于文燕%孫湛
마기%마소연%요설평%우문연%손담
黑木耳%脓毒血症%肿瘤坏死因子%白介素-1%白介素-6%核转录因子
黑木耳%膿毒血癥%腫瘤壞死因子%白介素-1%白介素-6%覈轉錄因子
흑목이%농독혈증%종류배사인자%백개소-1%백개소-6%핵전록인자
Auricularia auricula-judae%sepsis%tumor necrosis factor (TNF-α)%interleukin-1 (IL-1)%inter-leukin-6 (IL-6)%nuclear factor-κB (NF-κB)
目的探讨黑木耳提取物对脓毒血症大鼠血清中内毒素、肿瘤坏死因子（TNF-α）、白介素-1（IL-1）、白介素-6（IL-6）水平及肠、肝组织中核转录因子-κB（NF-κB）表达的影响。方法将健康Wistar大鼠随机分为对照组、实验组、蒙脱石散组、黑木耳多糖组、黑木耳粗提物组，术前分别预防性灌胃3d。第4天行手术，使用盲肠结扎穿刺法制备脓毒血症动物模型，造模12h后分别于门静脉和腹主动脉取血，检测血清内毒素水平；应用ELISA试剂盒检测血清中TNF-α、IL-1、IL-6水平；采用免疫组织化学法检测肝、肠组织细胞中NF-κB的表达水平。结果实验组、蒙脱石散组、黑木耳粗提物组及黑木耳多糖组门静脉血清和动脉血清内毒素水平均高于对照组，差异有统计学意义（P＜0．05）；黑木耳粗提物组及黑木耳多糖组门静脉血清和动脉血清内毒素水平均明显低于实验组及蒙脱石散组，差异有统计学意义（P＜0．05）。实验组、蒙脱石散组、黑木耳粗提物组及黑木耳多糖组大鼠血清中TNF-α、IL-1、IL-6水平较对照组升高，差异有统计学意义（P＜0．05）；蒙脱石散组、黑木耳粗提物组及黑木耳多糖组大鼠血清中TNF-α、IL-1较实验组降低，差异有统计学意义（P＜0．05）。实验组大鼠肝、肠组织细胞NF-κB强阳性表达，蒙脱石散组、黑木耳粗提物组和黑木耳多糖组肝、肠组织细胞NF-κB弱阳性表达，通过半定量测定，蒙脱石散组、黑木耳粗提物组和黑木耳多糖组肝、肠细胞NF-κB表达水平较实验组降低，差异有统计学意义（P＜0．01）。结论黑木耳提取物在有效降低脓毒血症大鼠血清中内毒素的基础上，同时降低TNF-α、IL-1、IL-6含量，抑制脓毒血症大鼠肝、肠组织细胞NF-κB的表达。
目的探討黑木耳提取物對膿毒血癥大鼠血清中內毒素、腫瘤壞死因子（TNF-α）、白介素-1（IL-1）、白介素-6（IL-6）水平及腸、肝組織中覈轉錄因子-κB（NF-κB）錶達的影響。方法將健康Wistar大鼠隨機分為對照組、實驗組、矇脫石散組、黑木耳多糖組、黑木耳粗提物組，術前分彆預防性灌胃3d。第4天行手術，使用盲腸結扎穿刺法製備膿毒血癥動物模型，造模12h後分彆于門靜脈和腹主動脈取血，檢測血清內毒素水平；應用ELISA試劑盒檢測血清中TNF-α、IL-1、IL-6水平；採用免疫組織化學法檢測肝、腸組織細胞中NF-κB的錶達水平。結果實驗組、矇脫石散組、黑木耳粗提物組及黑木耳多糖組門靜脈血清和動脈血清內毒素水平均高于對照組，差異有統計學意義（P＜0．05）；黑木耳粗提物組及黑木耳多糖組門靜脈血清和動脈血清內毒素水平均明顯低于實驗組及矇脫石散組，差異有統計學意義（P＜0．05）。實驗組、矇脫石散組、黑木耳粗提物組及黑木耳多糖組大鼠血清中TNF-α、IL-1、IL-6水平較對照組升高，差異有統計學意義（P＜0．05）；矇脫石散組、黑木耳粗提物組及黑木耳多糖組大鼠血清中TNF-α、IL-1較實驗組降低，差異有統計學意義（P＜0．05）。實驗組大鼠肝、腸組織細胞NF-κB彊暘性錶達，矇脫石散組、黑木耳粗提物組和黑木耳多糖組肝、腸組織細胞NF-κB弱暘性錶達，通過半定量測定，矇脫石散組、黑木耳粗提物組和黑木耳多糖組肝、腸細胞NF-κB錶達水平較實驗組降低，差異有統計學意義（P＜0．01）。結論黑木耳提取物在有效降低膿毒血癥大鼠血清中內毒素的基礎上，同時降低TNF-α、IL-1、IL-6含量，抑製膿毒血癥大鼠肝、腸組織細胞NF-κB的錶達。
목적탐토흑목이제취물대농독혈증대서혈청중내독소、종류배사인자（TNF-α）、백개소-1（IL-1）、백개소-6（IL-6）수평급장、간조직중핵전록인자-κB（NF-κB）표체적영향。방법장건강Wistar대서수궤분위대조조、실험조、몽탈석산조、흑목이다당조、흑목이조제물조，술전분별예방성관위3d。제4천행수술，사용맹장결찰천자법제비농독혈증동물모형，조모12h후분별우문정맥화복주동맥취혈，검측혈청내독소수평；응용ELISA시제합검측혈청중TNF-α、IL-1、IL-6수평；채용면역조직화학법검측간、장조직세포중NF-κB적표체수평。결과실험조、몽탈석산조、흑목이조제물조급흑목이다당조문정맥혈청화동맥혈청내독소수평균고우대조조，차이유통계학의의（P＜0．05）；흑목이조제물조급흑목이다당조문정맥혈청화동맥혈청내독소수평균명현저우실험조급몽탈석산조，차이유통계학의의（P＜0．05）。실험조、몽탈석산조、흑목이조제물조급흑목이다당조대서혈청중TNF-α、IL-1、IL-6수평교대조조승고，차이유통계학의의（P＜0．05）；몽탈석산조、흑목이조제물조급흑목이다당조대서혈청중TNF-α、IL-1교실험조강저，차이유통계학의의（P＜0．05）。실험조대서간、장조직세포NF-κB강양성표체，몽탈석산조、흑목이조제물조화흑목이다당조간、장조직세포NF-κB약양성표체，통과반정량측정，몽탈석산조、흑목이조제물조화흑목이다당조간、장세포NF-κB표체수평교실험조강저，차이유통계학의의（P＜0．01）。결론흑목이제취물재유효강저농독혈증대서혈청중내독소적기출상，동시강저TNF-α、IL-1、IL-6함량，억제농독혈증대서간、장조직세포NF-κB적표체。
Objective To discuss the effects of A .auricula-judae extract on rat model of sepsis ,this study mainly examines the serum concentration of endotoxin ,tumor necrosis factor (TNF-α) ,interleukin-1 (IL-1) , interleukin-6 (IL-6) and nuclear factor-κB (NF-κB) expression in intestinal and liver tissue .Methods 50 Wistar rats were randomized into 5 groups :sham surgery control group ,experiment group ,montmorillon-ite powder group ,A .auricula-judae polysaccharide group and crude A .auricula-judae extract group .Intra-gastric administration was performed every 8 hours three days prior to the surgery (1 .5 mL/100 g) .Sepsis models were induced by cecal ligation and puncture (CLP) procedure .Blood was collected from portal vein and abdominal aorta ,endotoxin concentration was detected by UV-Vis ;ELISA test was performed to de-termine the serum level of TNF-α,IL-1 ,IL-6 ;and immunohistochemistry was processed to detect NF-κB expression in intestinal and liver tissue .Results Two A .auricula-judae groups showed a much lower con-centration of serum endotoxin than the experiment group and montmorillonite powder group (P <0 .01);while the differences between the experiment group and montmorillonite group showed no statistical signif-icance (P >0 .05) .As to the three cytokines ,the montmorillonite powder group and two A .auricula-judae groups all presented lower level of the examined cytokines than the experiment group (P <0 .05);while the differences between montmorillonite powder and two A .auricula-judae groups showed no statistical sig-nificance (P >0 .05) .Unlike a weak staining detected in the control group ,experiment group showed a strong positive NF-κB staining (P < 0 .01);the staining in montmorillonite powder group was positive , and a moderate staining was detected in the two A .auricula-judae groups .Conclusion A .auricula-judae extract effectively decreases serum endotoxin concentration as well as serum level of TNF-α,IL-1 ,IL-6 in sepsis models ;it also prohibits the activation of NF-κB in intestinal and liver tissue of sepsis rats .
