CAJ | 학술논문

目的探讨分析新疆哈萨克族食管癌患者食管癌组织及癌旁组织中金属硫蛋白3（metallothionein-3， MT-3）基因CpG岛的甲基化状态及其与食管癌发生的关系。方法采用甲基化特异性聚合酶链反应（MSP）的方法，检测33例哈萨克族食管癌患者食管癌组织和癌旁组织中金属硫蛋白3（M T-3）基因启动子区C pG岛的甲基化发生状况。结果33例哈萨克族食管癌患者癌组织和癌旁组织标本中M T-3基因启动子区C pG岛甲基化的发生率分别为66．7％（22/33）和27．3％（9/33）；癌组织与癌旁组织甲基化率差异有统计学意义（χ2＝10．28， P ＝0．001）。结论 M T-3基因的甲基化可能与食管癌的发生与发展有关。
목적탐토분석신강합살극족식관암환자식관암조직급암방조직중금속류단백3（metallothionein-3， MT-3）기인CpG도적갑기화상태급기여식관암발생적관계。방법채용갑기화특이성취합매련반응（MSP）적방법，검측33례합살극족식관암환자식관암조직화암방조직중금속류단백3（M T-3）기인계동자구C pG도적갑기화발생상황。결과33례합살극족식관암환자암조직화암방조직표본중M T-3기인계동자구C pG도갑기화적발생솔분별위66．7％（22/33）화27．3％（9/33）；암조직여암방조직갑기화솔차이유통계학의의（χ2＝10．28， P ＝0．001）。결론 M T-3기인적갑기화가능여식관암적발생여발전유관。
Objective To explore the relationship between the level of Methylation of the CpG island in the M T-3 gene in esophageal carcinoma in the Kazak .Methods The methylation of M T-3 gene was detected by methylation specific polymerase chain reaction (MSP) in 33 esophageal cancer tissues and adjacent tis-sues .Results T he methylation rates of the M T-3 gene in esophageal cancer tissues and adjacent tissues were 66 .7% and 27 .3% in Kazaks ,Significant differences were seen in the methylation rates of M T-3 gene in esophageal cancer tissues and adjacent tissues (χ2 =10 .28 ,P=0 .001) .Conclusion Aberrant promoter methylation of the CpG island in the M T-3 gene maybe play an important role in the pathogenesis of esoph-ageal squamous cell carcinoma in the Kazak .