CAJ | 학술논문

目的探讨不同感染泡球蚴动物模型的方法，找出适合肝移植的最佳感染模型。方法将实验大鼠分为A组（皮下注射接种感染组）、B组（开腹直视肝穿刺注射感染组）、C组（经皮肝脏穿刺接种感染组）。感染3个月后观察各组大鼠的感染率、死亡率，ELISA检测 Th1类细胞因子干扰素γ（interferon-γ，IFN-γ）和 Th2类细胞因子白介素10（interleukin-10，IL-10）在血浆中的表达情况。结果 ELISA检测3种方法感染大鼠外周血中 IFN-γ、IL-10的浓度差异无统计学意义（P ＜0．05）。皮下注射泡球蚴成功建立了 E ．m感染大鼠模型，其中死亡0只，死亡率0％（0/20），感染率60％（12/20）。结论皮下感染泡球蚴的动物模型更适合肝移植动物模型的建立。
목적탐토불동감염포구유동물모형적방법，조출괄합간이식적최가감염모형。방법장실험대서분위A조（피하주사접충감염조）、B조（개복직시간천자주사감염조）、C조（경피간장천자접충감염조）。감염3개월후관찰각조대서적감염솔、사망솔，ELISA검측 Th1류세포인자간우소γ（interferon-γ，IFN-γ）화 Th2류세포인자백개소10（interleukin-10，IL-10）재혈장중적표체정황。결과 ELISA검측3충방법감염대서외주혈중 IFN-γ、IL-10적농도차이무통계학의의（P ＜0．05）。피하주사포구유성공건립료 E ．m감염대서모형，기중사망0지，사망솔0％（0/20），감염솔60％（12/20）。결론피하감염포구유적동물모형경괄합간이식동물모형적건립。
Objectives To explore various approach for murine alveolar echinococcosis model and to find the most feasible approach .Methods Experimental murine was divided into three groups .Group A :intrader-mal approach ;Group B :direct transhepatic approach ;Group C :percutaneous transhepatic approach . Mortality and infection rate were observed in postinfection 3 months in all groups .Serum level of Th1 re-lated cytokine IFN-γ and Th2 related cytokine IL-10 were detected by ELISA .Results Infection rate in intradermal approach was 60 % (12/20) ,with no mortality observed .However ,no significant difference was observed interm of serum IFN-γand IL-10 levels (P <0 .05) .Intradermal approach seem to be more suitable for liver transplantation model in AE murine .Conclusion Subcutaneous infection echinococcus multilocularis of animal model is more suitable for the establishment of animal model of liver transplanta-tio n .