世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2013年
9期
1053-1057
,共5页
丛晨阳%温莹%毕宏生%解孝锋%郭大东%杜然然
叢晨暘%溫瑩%畢宏生%解孝鋒%郭大東%杜然然
총신양%온형%필굉생%해효봉%곽대동%두연연
参麦合剂%兔%干眼症%结膜印记细胞学检查
參麥閤劑%兔%榦眼癥%結膜印記細胞學檢查
삼맥합제%토%간안증%결막인기세포학검사
Shenmai-mixture%Rabbit%Dry eye%Conjunctival imprint cytology
目的:探索参麦合剂对兔干眼症模型泪液分泌及眼表结构的影响。方法:将30只健康新西兰大白兔随机分为空白对照组、实验对照组、药物治疗组。制作干眼动物模型后,各组给予相应治疗。治疗前和治疗后第7、14、21、28 d分别测定Schirmer试验值,BUT值、眼表荧光素染色( FL)、结膜印记细胞学检查( CIC),观察眼表泪膜状态和细胞学变化。治疗28 d后,每组随机选取5只动物,处死后剪下角结膜组织,行角结膜石蜡切片光镜观察。结果:药物治疗组用药14 d后,Schirmer值及BUT值均较其他两组明显增加( P<0.01);结膜细胞印迹学检查发现药物治疗组结膜杯状细胞数量较其他两组明显增多;光镜检查发现药物治疗组与其他两组相比,部分结膜上皮层数增多、杯状细胞增多。结论:参麦合剂能明显增加兔干眼症模型泪液分泌、改善泪膜稳定性和眼表细胞结构。
目的:探索參麥閤劑對兔榦眼癥模型淚液分泌及眼錶結構的影響。方法:將30隻健康新西蘭大白兔隨機分為空白對照組、實驗對照組、藥物治療組。製作榦眼動物模型後,各組給予相應治療。治療前和治療後第7、14、21、28 d分彆測定Schirmer試驗值,BUT值、眼錶熒光素染色( FL)、結膜印記細胞學檢查( CIC),觀察眼錶淚膜狀態和細胞學變化。治療28 d後,每組隨機選取5隻動物,處死後剪下角結膜組織,行角結膜石蠟切片光鏡觀察。結果:藥物治療組用藥14 d後,Schirmer值及BUT值均較其他兩組明顯增加( P<0.01);結膜細胞印跡學檢查髮現藥物治療組結膜杯狀細胞數量較其他兩組明顯增多;光鏡檢查髮現藥物治療組與其他兩組相比,部分結膜上皮層數增多、杯狀細胞增多。結論:參麥閤劑能明顯增加兔榦眼癥模型淚液分泌、改善淚膜穩定性和眼錶細胞結構。
목적:탐색삼맥합제대토간안증모형루액분비급안표결구적영향。방법:장30지건강신서란대백토수궤분위공백대조조、실험대조조、약물치료조。제작간안동물모형후,각조급여상응치료。치료전화치료후제7、14、21、28 d분별측정Schirmer시험치,BUT치、안표형광소염색( FL)、결막인기세포학검사( CIC),관찰안표루막상태화세포학변화。치료28 d후,매조수궤선취5지동물,처사후전하각결막조직,행각결막석사절편광경관찰。결과:약물치료조용약14 d후,Schirmer치급BUT치균교기타량조명현증가( P<0.01);결막세포인적학검사발현약물치료조결막배상세포수량교기타량조명현증다;광경검사발현약물치료조여기타량조상비,부분결막상피층수증다、배상세포증다。결론:삼맥합제능명현증가토간안증모형루액분비、개선루막은정성화안표세포결구。
Objective:To explore the effects of Shenmai-mixture on tear secretion and the ocular surface structure of rabbit dry eye mod-el.Methods:Thirty healthy New Zealand white rabbits were divided randomly into 3 groups:control group, experimental control group and drug-treated group .After the rabbit dry eye model was established , the control group were not interfered , the experimental control group received saline intragastric administration , the drug treatment group received Shenmai mixture intragastric administration .On day 0,7,14,21 and 28, Schirmer test, tear break-up time(BUT), fluorescein staining of the ocular surface were measured and conjunctival impression cytology specimens were collected .All of these were used to observe the state of the tear film and ocular surface changes . Subjects underwent conjunctival imprint cytology inspection to observe the ocular surface change in cytology .On day 28, cornea and con-junctiva were harvested for Hematoxylin and Eosin (HE) staining, scanning observation.Results:The Schirmer test value and BUT val-ue in drug-treated group were significantly longer than those in other two groups on day 14(P<0.01).After intragastric administration of Shenmai-mixture for 28 days,c onjunctival imprint cytology showed the conjunctival goblet cells in the drug -treated group increased significantly than the other two groups .Epithelial layers and conjunctival goblet cells increased as shown by the HE staining .Conclu-sion:Shenmai-mixture can be used to treat dry eyes because it increase tear secration , improves stability of tear and change the structure of ocular surface .