CAJ | 학술논문

目的：评价不同结核分枝杆菌核酸（TB-DNA ）试剂盒的性能和应用。方法本文通过采用中山达安、德国凯杰及上海之江TB-DNA检测试剂盒平行检测抗酸染色阳性的临床标本，并用非结核病患者标本来验证假阳性率。结果44例抗酸染色阳性标本中，中山达安试剂盒检测出44例TB-DNA阳性，德国凯杰试剂盒检测出43例阳性，上海之江试剂盒检测出40例阳性；3种试剂盒检测20例非结核患者的标本，TB-DNA均为阴性。3种试剂盒的阳性检出率分别为100％、97．7％和90．9％，差异无统计学意义（χ2＝5．40，P＞0．05）。结论各实验室可灵活选择临床检测试剂盒。
목적：평개불동결핵분지간균핵산（TB-DNA ）시제합적성능화응용。방법본문통과채용중산체안、덕국개걸급상해지강TB-DNA검측시제합평행검측항산염색양성적림상표본，병용비결핵병환자표본래험증가양성솔。결과44례항산염색양성표본중，중산체안시제합검측출44례TB-DNA양성，덕국개걸시제합검측출43례양성，상해지강시제합검측출40례양성；3충시제합검측20례비결핵환자적표본，TB-DNA균위음성。3충시제합적양성검출솔분별위100％、97．7％화90．9％，차이무통계학의의（χ2＝5．40，P＞0．05）。결론각실험실가령활선택림상검측시제합。
Objective To evaluate the difference of three Mycobacterium tuberculosis detection kits and provide experimental ba-sis for clinical applications .Methods Three kinds of Mycobacterium tuberculosis detection kits which named Zhongshan Da′an , Germany Qiagen and Shanghai Zhijiang were used to detect Mycobacterium tuberculosis DNA(TB-DNA) in clinical specimens . Acid-fast staining was positive ,and specimens in patients without tuberculosis infection were used to verify the false positive .Re-sults The positive rate of Zhongshan Da′an ,Germany Qiagen and Shanghai Zhijiang detection kits were 100% (44/44) ,97 .7% (43/44) and 90 .9% (40/44) ,respectively ,and the difference among those three kits were not statistically significant (χ2 = 5 .40 ,P>0 .05) .TB-DNA detection by using the three kinds of kits in 20 cases of non-tuberculosis infection specimens were all negative .Con-clusion Clinical laboratory might choose anyone of these TB-DNA detection kits to the diagnose Mycobacterium tuberculosis .