中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2014年
14期
8-11,15
,共5页
刘丽娜%殷云勤%张兆美%席玉
劉麗娜%慇雲勤%張兆美%席玉
류려나%은운근%장조미%석옥
结直肠肿瘤%AGR2%Lgr5%免疫组织化学
結直腸腫瘤%AGR2%Lgr5%免疫組織化學
결직장종류%AGR2%Lgr5%면역조직화학
Colorectal cancer%AGR2%Lgr5%Immunohistochemistry
目的：探讨AGR2和Lgr5在人CRC中表达及其与临床病理特征的关系，评价二者联合检测对CRC诊断的意义。方法采用免疫组化SABC法检测70例CRC组织和20例癌旁正常组织中AGR2和Lgr5的表达情况。结果①CRC组织中AGR2阳性表达率较癌旁正常组织显著性增高（68.57％、25.00％，P﹤0.01）。在CRC组织中， AGR2的阳性表达率与肿瘤分化程度与淋巴结转移呈显著相关（P﹤0.05）。②CRC组织中Lgr5阳性表达率较癌旁正常组织显著性增高（74.29％、40.00％，P﹤0.01）。在CRC组织中，Lgr5的阳性表达率与肿瘤分化程度、浸润深度和淋巴结转移有显著相关性（P﹤0.05）。③联合检测AGR2、Lgr5曲线下面积与单独检测比较，差异有统计学意义（P均﹤0.05）。结论 AGR2、Lgr5在CRC的发生、发展过程中发挥了重要作用，两者联合检测有助于对CRC患者肿瘤进展的判断，为CRC患者的分子诊断和治疗提供了理论支持。
目的：探討AGR2和Lgr5在人CRC中錶達及其與臨床病理特徵的關繫，評價二者聯閤檢測對CRC診斷的意義。方法採用免疫組化SABC法檢測70例CRC組織和20例癌徬正常組織中AGR2和Lgr5的錶達情況。結果①CRC組織中AGR2暘性錶達率較癌徬正常組織顯著性增高（68.57％、25.00％，P﹤0.01）。在CRC組織中， AGR2的暘性錶達率與腫瘤分化程度與淋巴結轉移呈顯著相關（P﹤0.05）。②CRC組織中Lgr5暘性錶達率較癌徬正常組織顯著性增高（74.29％、40.00％，P﹤0.01）。在CRC組織中，Lgr5的暘性錶達率與腫瘤分化程度、浸潤深度和淋巴結轉移有顯著相關性（P﹤0.05）。③聯閤檢測AGR2、Lgr5麯線下麵積與單獨檢測比較，差異有統計學意義（P均﹤0.05）。結論 AGR2、Lgr5在CRC的髮生、髮展過程中髮揮瞭重要作用，兩者聯閤檢測有助于對CRC患者腫瘤進展的判斷，為CRC患者的分子診斷和治療提供瞭理論支持。
목적：탐토AGR2화Lgr5재인CRC중표체급기여림상병리특정적관계，평개이자연합검측대CRC진단적의의。방법채용면역조화SABC법검측70례CRC조직화20례암방정상조직중AGR2화Lgr5적표체정황。결과①CRC조직중AGR2양성표체솔교암방정상조직현저성증고（68.57％、25.00％，P﹤0.01）。재CRC조직중， AGR2적양성표체솔여종류분화정도여림파결전이정현저상관（P﹤0.05）。②CRC조직중Lgr5양성표체솔교암방정상조직현저성증고（74.29％、40.00％，P﹤0.01）。재CRC조직중，Lgr5적양성표체솔여종류분화정도、침윤심도화림파결전이유현저상관성（P﹤0.05）。③연합검측AGR2、Lgr5곡선하면적여단독검측비교，차이유통계학의의（P균﹤0.05）。결론 AGR2、Lgr5재CRC적발생、발전과정중발휘료중요작용，량자연합검측유조우대CRC환자종류진전적판단，위CRC환자적분자진단화치료제공료이론지지。
Objective To investigate the expression and clinical significance of AGR2 and Lgr5 in CRC and the corre-lation with clinical pathologic features. To evaluate the significance with combined detection of the clinical and patho-logical diagnosis of CRC. Methods AGR2 and Lgr5 expression were assessed in 70 cases of CRC and 20 cases of nor-mal tissues near the cancer by immunohistochemistry. Results ①The expression of AGR2 was significantly higher in CRC than that in normal tissue(68.57%vs 25.00%,P<0.01). The AGR2 expression in CRC was correlated with the de-gree of tumor differentiation and lymph node metastasis(P<0.05).②The expression of Lgr5 was significantly higher in CRC than that in normal tissue (74.29%vs 40.00%, P<0.01). The Lgr5 expression in CRC was correlated with the de-gree of tumor differentiation﹑depth of invasion and lymph node metastasis(P<0.05). ③The Area Under Curve(AUC) of combined detection of AGR2 and Lgr5 were significantly higher than detection of both alone. the difference was statis-tically significant (P all <0.05). Conclusion AGR2 and Lgr5 played an important role in the occurrence and develop-ment of CRC, the combined detection not only contribute to the judgment of tumor progression, but also provide theo-retical support for molecular diagnosis and treatment of patients with CRC .
