西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2013年
9期
42-44
,共3页
十味黑冰片丸%没食子酸%反相高效液相色谱法
十味黑冰片汍%沒食子痠%反相高效液相色譜法
십미흑빙편환%몰식자산%반상고효액상색보법
ShiWei HeiBingPianWan%gallic acid%RP-HPLC
目的：以没食子酸含量建立十味黑冰片丸的质量控制标准。方法：采用反相高效液相色谱法，用十八烷基硅烷键合硅胶柱，流动相为乙腈-4%磷酸溶液(5∶95)，检测波长为272 nm ，流速0.8 mL/min。结果：没食子酸对照品在0.4～2.0μg浓度内线性关系良好，r=0.9993。平均加样回收率99.64%， RSD为0.22%(n=6)。结论：该方法准确可靠、简便，可用于十味黑冰片丸的质量控制。
目的：以沒食子痠含量建立十味黑冰片汍的質量控製標準。方法：採用反相高效液相色譜法，用十八烷基硅烷鍵閤硅膠柱，流動相為乙腈-4%燐痠溶液(5∶95)，檢測波長為272 nm ，流速0.8 mL/min。結果：沒食子痠對照品在0.4～2.0μg濃度內線性關繫良好，r=0.9993。平均加樣迴收率99.64%， RSD為0.22%(n=6)。結論：該方法準確可靠、簡便，可用于十味黑冰片汍的質量控製。
목적：이몰식자산함량건립십미흑빙편환적질량공제표준。방법：채용반상고효액상색보법，용십팔완기규완건합규효주，류동상위을정-4%린산용액(5∶95)，검측파장위272 nm ，류속0.8 mL/min。결과：몰식자산대조품재0.4～2.0μg농도내선성관계량호，r=0.9993。평균가양회수솔99.64%， RSD위0.22%(n=6)。결론：해방법준학가고、간편，가용우십미흑빙편환적질량공제。
Objective:To establish a method for quality control of ShiWei HeiBingPianWan by determining the contents of gallic acid. Method:Reversed phase high-performance liquid chromatography (RP-HPLC) was adopted, the C18 column consisted of acetonitrile-4% Phosphoric acid (5:95) as mobile phase, 272nm as detection wave-length, 0.8mL/min as flow rate. Result:Gallic acid showed a better linear relationship in the range of 0.4~2.0 μg, r=0.999 3. Average recovery rate was 99.64%, RSD was 0.22% (n=6). Conclusion:The method, accurate, reliable and simple, could be used for quality control of ShiWei HeiBingPianWan.
