CAJ | 학술논문

目的：探讨止消通脉宁对转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)Smad 2、Smad 3、Smad 7的影响。方法：将HK-2细胞用含10%胎牛血清的DMEM/F12(1：1)培养基培养；实验分为6组：空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、中药含药血清低剂量组(TGF-β110 ng/mL+10%低剂量止消通脉宁含药血清)、中药含药血清中剂量组(TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、中药含药血清高剂量组(TGF-β110 ng/mL+10%高剂量止消通脉宁含药血清)。药物干预24小时后，荧光定量PCR检测Smad 2、smad 3、Smad 7的mRNA表达。结果：HK-2细胞经TGF-β1诱导后， Smad 2、smad 3的mRNA表达显著上升，Smad 7的mRNA表达下降，与空白对照组相比有显著性差异(P＜0.05)，但经止消通脉宁含药血清干预后， Smad 2、smad 3的mRNA表达逐步下降，Smad 7的mRNA表达上调，与单纯TGF-β1诱导组相比有显著性差异(P＜0.05)。而空白血清无此作用。结论：止消通脉宁能够调控TGF-β1诱导的人肾小管上皮细胞转分化Smad信号通路mRNA表达的作用，在一定程度上具有抑制肾间质纤维化的作用。
목적：탐토지소통맥저대전화생장인자-β1(TGF-β1)유도적인신소관상피세포(HK-2)Smad 2、Smad 3、Smad 7적영향。방법：장HK-2세포용함10%태우혈청적DMEM/F12(1：1)배양기배양；실험분위6조：공백대조조、단순TGF-β1유도조(TGF-β110 ng/mL)、공백혈청대조조(TGF-β110 ng/mL+10%공백혈청)、중약함약혈청저제량조(TGF-β110 ng/mL+10%저제량지소통맥저함약혈청)、중약함약혈청중제량조(TGF-β110 ng/mL+10%중제량지소통맥저함약혈청)、중약함약혈청고제량조(TGF-β110 ng/mL+10%고제량지소통맥저함약혈청)。약물간예24소시후，형광정량PCR검측Smad 2、smad 3、Smad 7적mRNA표체。결과：HK-2세포경TGF-β1유도후， Smad 2、smad 3적mRNA표체현저상승，Smad 7적mRNA표체하강，여공백대조조상비유현저성차이(P＜0.05)，단경지소통맥저함약혈청간예후， Smad 2、smad 3적mRNA표체축보하강，Smad 7적mRNA표체상조，여단순TGF-β1유도조상비유현저성차이(P＜0.05)。이공백혈청무차작용。결론：지소통맥저능구조공TGF-β1유도적인신소관상피세포전분화Smad신호통로mRNA표체적작용，재일정정도상구유억제신간질섬유화적작용。
Objective:To explore the effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 of hu-man renal tubular epithelial cells (HK-2) which were induced by transforming growth factor (TGF-β1). Method:HK-2 cells were cultured in the media of DMEM/F12 (1:1) containing 10%fetal bovine serum;the cells were divid-ed into six groups:blank control group, TGF-β1 group (TGF-β110 ng/mL), blank serum control group (TGF-β1 10 ng/mL+10%blank serum), low dose group of TCM medicated serum (TGF-β110 ng/mL+10%low dose medicated serum of ZhiXiao TongMaiNing), moderate dose group of TCM medicated serum (TGF-β1 10 ng/mL+10%moderate dose medicated serum of ZhiXiao TongMaiNing) and high dose group of TCM medicated serum (TGF-β1 10 ng/mL+10%high dose medicated serum of ZhiXiao TongMaiNing). After intervened for 24 hours, Smad 2, Smad 3 and Smad 7 mRNA expressions were detected with fluorescence quantitative PCR. Result:Smad2 and Smad3 mR-NA expressions were raised significantly, Smad 7 mRNA expressions were decreased when HK-2 cells were in-duced with TGF-β1, it had significant difference compared with blank control group (P<0.05), but after intervened with medicated serum of ZhiXiao TongMaiNing, Smad 2 and Smad 3 mRNA expressions were lowered gradually, Smad 7 mRNA expressions were improved, it had statistical meaning compared with TGF-β1 group (P<0.05). Blank serum presented no effects. Conclusion:ZhiXiao TongMaiNing could regulate mRNA expressions of trans-differenti-ated Smad signal channel of HK-2 cells induced with TGF-β1 and inhibit the fibrosis of renal tubular epithelial cells to a certain extent.