CAJ | 학술논문

目的：通过观察健膝丸对膝骨关节炎模型SD大鼠血清SOD、MDA、NO表达水平的影响，以探求健膝丸在防治膝骨关节炎方面的作用机理，从而为本药的临床应用及药物开发提供科学的理论依据，同时进一步探索骨关节炎的发病机制。方法：以40只雌性SD大鼠为实验对象，随机分为正常组、模型对照组、健膝丸组、抗骨增生胶囊组，每组10只。除正常组外，其余各组均采用改良Hulth法对大鼠进行造模。术后连续3 d肌肉注射青霉素20万U，1周后开始强迫大鼠每天活动0.5 h，连续8周。同时各组大鼠均采用灌胃给药途径给予相应药液，根据人与动物体表面积等效剂量折算出健膝丸组及抗骨增生胶囊组灌胃给药量。正常组及模型对照组给予质量浓度0.9％的生理盐水，连续8周。8周后分别抽取各组大鼠腹腔静脉血，离心后取上清，按照SOD、MDA、NO测试盒说明操作，用754分光光度计测各管吸光度（OD值），从而进一步计算出各组血清中的各指标的含量。结果：血清中SOD、MDA、NO含量测定结果显示，模型对照组与正常组比较，模型对照组血清中SOD活性显著下降（P<0.01），MDA、NO含量显著升高（P<0.01）。健膝丸组与正常组比较，SOD活性差异无统计学意义（P>0.05），MDA及NO含量差异有统计学意义（P<0.05）。健膝丸组与模型对照组比较，SOD活性显著升高（P<0.01），MDA含量差异无统计学意义（P>0.05）、NO含量显著下降（P<0.01）。对SOD、MDA、NO含量的影响，健膝丸组与抗骨增生胶囊组比较，差异无统计学意义（P>0.05）。结论：通过对实验各组SD大鼠血清中SOD、MDA、NO水平的检测，结果显示，在膝骨关节炎模型SD大鼠血清中, SOD活性下降，MDA及NO含量升高，由此可知氧自由基等因子在骨关节炎的发病及软骨细胞的破坏进程中起到重要作用。健膝丸对血清MDA的含量影响不明显，但可显著提高SOD活性、清除体内过量的氧自由基，同时抑制了NO的过度产生、降低其生物效应，改善关节微循环、保护软骨细胞或促使受损细胞的修复，从而起到延缓关节软骨退变的作用。目的：通過觀察健膝汍對膝骨關節炎模型SD大鼠血清SOD、MDA、NO錶達水平的影響，以探求健膝汍在防治膝骨關節炎方麵的作用機理，從而為本藥的臨床應用及藥物開髮提供科學的理論依據，同時進一步探索骨關節炎的髮病機製。方法：以40隻雌性SD大鼠為實驗對象，隨機分為正常組、模型對照組、健膝汍組、抗骨增生膠囊組，每組10隻。除正常組外，其餘各組均採用改良Hulth法對大鼠進行造模。術後連續3 d肌肉註射青黴素20萬U，1週後開始彊迫大鼠每天活動0.5 h，連續8週。同時各組大鼠均採用灌胃給藥途徑給予相應藥液，根據人與動物體錶麵積等效劑量摺算齣健膝汍組及抗骨增生膠囊組灌胃給藥量。正常組及模型對照組給予質量濃度0.9％的生理鹽水，連續8週。8週後分彆抽取各組大鼠腹腔靜脈血，離心後取上清，按照SOD、MDA、NO測試盒說明操作，用754分光光度計測各管吸光度（OD值），從而進一步計算齣各組血清中的各指標的含量。結果：血清中SOD、MDA、NO含量測定結果顯示，模型對照組與正常組比較，模型對照組血清中SOD活性顯著下降（P<0.01），MDA、NO含量顯著升高（P<0.01）。健膝汍組與正常組比較，SOD活性差異無統計學意義（P>0.05），MDA及NO含量差異有統計學意義（P<0.05）。健膝汍組與模型對照組比較，SOD活性顯著升高（P<0.01），MDA含量差異無統計學意義（P>0.05）、NO含量顯著下降（P<0.01）。對SOD、MDA、NO含量的影響，健膝汍組與抗骨增生膠囊組比較，差異無統計學意義（P>0.05）。結論：通過對實驗各組SD大鼠血清中SOD、MDA、NO水平的檢測，結果顯示，在膝骨關節炎模型SD大鼠血清中, SOD活性下降，MDA及NO含量升高，由此可知氧自由基等因子在骨關節炎的髮病及軟骨細胞的破壞進程中起到重要作用。健膝汍對血清MDA的含量影響不明顯，但可顯著提高SOD活性、清除體內過量的氧自由基，同時抑製瞭NO的過度產生、降低其生物效應，改善關節微循環、保護軟骨細胞或促使受損細胞的脩複，從而起到延緩關節軟骨退變的作用。
목적：통과관찰건슬환대슬골관절염모형SD대서혈청SOD、MDA、NO표체수평적영향，이탐구건슬환재방치슬골관절염방면적작용궤리，종이위본약적림상응용급약물개발제공과학적이론의거，동시진일보탐색골관절염적발병궤제。방법：이40지자성SD대서위실험대상，수궤분위정상조、모형대조조、건슬환조、항골증생효낭조，매조10지。제정상조외，기여각조균채용개량Hulth법대대서진행조모。술후련속3 d기육주사청매소20만U，1주후개시강박대서매천활동0.5 h，련속8주。동시각조대서균채용관위급약도경급여상응약액，근거인여동물체표면적등효제량절산출건슬환조급항골증생효낭조관위급약량。정상조급모형대조조급여질량농도0.9％적생리염수，련속8주。8주후분별추취각조대서복강정맥혈，리심후취상청，안조SOD、MDA、NO측시합설명조작，용754분광광도계측각관흡광도（OD치），종이진일보계산출각조혈청중적각지표적함량。결과：혈청중SOD、MDA、NO함량측정결과현시，모형대조조여정상조비교，모형대조조혈청중SOD활성현저하강（P<0.01），MDA、NO함량현저승고（P<0.01）。건슬환조여정상조비교，SOD활성차이무통계학의의（P>0.05），MDA급NO함량차이유통계학의의（P<0.05）。건슬환조여모형대조조비교，SOD활성현저승고（P<0.01），MDA함량차이무통계학의의（P>0.05）、NO함량현저하강（P<0.01）。대SOD、MDA、NO함량적영향，건슬환조여항골증생효낭조비교，차이무통계학의의（P>0.05）。결론：통과대실험각조SD대서혈청중SOD、MDA、NO수평적검측，결과현시，재슬골관절염모형SD대서혈청중, SOD활성하강，MDA급NO함량승고，유차가지양자유기등인자재골관절염적발병급연골세포적파배진정중기도중요작용。건슬환대혈청MDA적함량영향불명현，단가현저제고SOD활성、청제체내과량적양자유기，동시억제료NO적과도산생、강저기생물효응，개선관절미순배、보호연골세포혹촉사수손세포적수복，종이기도연완관절연골퇴변적작용。
Objective:To probe action mechanism of jianxi pill on preventing knee osteoarthritis by observing its effects on superoxide dismutase(SOD),nitric oxide(NO)and malondialdehyde(MDA) of serum in rats with knee osteoarthritis to provide the scientiifc theory for its clinical practice and development and to further research the pathogenesis of osteoarthritis.Methods:40 SD female rats were randomly and equally divided into four groups: the normal group,the model control group,the jianxi pill group and the kanggu zengsheng capsule group.Except for the normal group,the other three groups were modeled by improved Hulth's technique.After operation,the rats were continuously injected with penicillin(200,000 U) for three days. One week later,the rats were forced to act for half an hour daily for eight weeks.In the meantime,all rats were given corresponding drugs by intragastric administration.According to the humanity and animal body surface area,the equivalent dose was worked out.The normal group and the model control group were continuously given 0.9% physiological saline for eight weeks.Eight weeks later,Rats' abdominal venous blood was extracted and centrifuged for blood serum.According to speciifcations,the content of SOD,MDA and NO were worked out with 754 spectrophotometer.Results:The assaying of SOD、MDA and NO in blood serum showed that compared with the normal control group,the activity of SOD in the model control groups decreased signiifcantly(P<0.01) and the content of MDA and NO increased signiifcantly(P<0.01).Compared with the normal group,the activity of SOD in the jianxi pill group had no obvious statistical difference(P>0.05) and the contents of MDA and NO had statistical difference(P<0.05).Compared with the model control group,the activity of SOD in the jianxi pill group increased signiifcantly(P<0.01) and the difference of the content of MDA had no obvious statistical difference(P>0.05),the content of NO in the group decreased significantly(P<0.01).The difference of the contents of SOD,MDA and NO between the jianxi pill group and the kanggu zengsheng capsule group had no statistical signiifcance(P>0.05).Conclusion:The result showed by measuring the contents of SOD,MDA and NO in rats' blood serum that the activity of SOD decreased and the contents of MDA and NO increased,by which we knew that the oxygen free radical played an important role in the onset of osteoarthritis and destruction of cartilage.Although jianxi pill had no obvious effect on the content of MDA,it could signiifcantly promote the activity of SOD and clear the oxygen free radical.In the meantime,it inhibited over generation of NO,decreasing its biological effect,improving joint's microcirculation,and protecting chondrocyte or promoting the restore of damaged cells,so to postpone the degeneration of articular cartilage.