CAJ | 학술논문

目的建立桃仁膝康丸高效液相色谱指纹图谱,提供较全面评价、控制制剂质量的方法。方法采用高效液相色谱-紫外检测法分析10批桃仁膝康丸的甲醇提取物。色谱柱Agilent C18(4.6 mm×250 mm,5μm)；流动相：乙腈-水梯度洗脱；流速：1.0 mL/min；检测波长：203 nm。结果建立了桃仁膝康丸高效液相色谱指纹图谱,共标示26个共有指纹峰,10批被测样品的色谱指纹图谱整体相似度在0.9以上。结论本方法准确可靠,可有效控制桃仁膝康丸的制剂质量。
목적건립도인슬강환고효액상색보지문도보,제공교전면평개、공제제제질량적방법。방법채용고효액상색보-자외검측법분석10비도인슬강환적갑순제취물。색보주Agilent C18(4.6 mm×250 mm,5μm)；류동상：을정-수제도세탈；류속：1.0 mL/min；검측파장：203 nm。결과건립료도인슬강환고효액상색보지문도보,공표시26개공유지문봉,10비피측양품적색보지문도보정체상사도재0.9이상。결론본방법준학가고,가유효공제도인슬강환적제제질량。
Objective To establish the HPLC fingerprint of Taoren Xikang pills and provide the method for the quality control and evaluation of the preparation. Methods Methanol extracts of ten batches of Taoren Xikang pills were analyzed with HPLC-UV method. Analysis was performed on C18 column (4.6 mm× 250 mm, 5 μm) with the mobile phase of acetonitrile-water gradient eluted at the flow rate of 1.0 mL/min. The detection wavelength was 203 nm. Results A standard HPLC fingerprint procedure was developed for Taoren Xikang pills with 26 common peaks. The similarity of 10 batches of Taoren Xikang pills was not lower than 0.9. Conclusion The method is accurate, repeatable and can be used for the quality control of Taoren Xikang pills.