CAJ | 학술논문

目的探讨亳芍生物碱提取、定性鉴别及含量测定方法。方法95%乙醇提取亳芍干粉，总提物回收乙醇后盐酸酸化，二氯甲烷萃取除杂，加氨水中和至弱碱性后氯仿萃取获总生物碱提物，薄层色谱检测，改良碘化铋钾等显色，酸碱滴定法测定总生物碱含量。结果总生物碱提取物经薄层色谱展开后，改良碘化铋钾显色，可检测到4个橙红色斑点；浓硫酸显色，其中2个斑点呈紫红色；甲醛浓硫酸显色，可见4个紫黑色斑点；以浓硝酸及茚三酮显色未见明显色斑。亳芍总生物碱含量约为0.079%。结论亳芍中含有生物碱，其中可能包含乌头碱类生物碱。
목적탐토박작생물감제취、정성감별급함량측정방법。방법95%을순제취박작간분，총제물회수을순후염산산화，이록갑완췌취제잡，가안수중화지약감성후록방췌취획총생물감제물，박층색보검측，개량전화필갑등현색，산감적정법측정총생물감함량。결과총생물감제취물경박층색보전개후，개량전화필갑현색，가검측도4개등홍색반점；농류산현색，기중2개반점정자홍색；갑철농류산현색，가견4개자흑색반점；이농초산급인삼동현색미견명현색반。박작총생물감함량약위0.079%。결론박작중함유생물감，기중가능포함오두감류생물감。
Objective To explore the extraction and assaying of the alkaloids from Bozhou Peony Paeonia lactiflora. Methods Dry root powder of the medical material was extracted by 90%ethanol. The extraction was first acidified by hydrochloric acid after recovering alcohol, and then extracted by dichloromethane. The acid solution was adjusted pH to alkalescence by ammonia water, then extracted by chloroform to get crude alkaloid. Bozhou Peony alkaloids were confirmed by TLC along with different colouration method, and its content was detected by acid-base titration. Results Four alkaloid-representative salmon patches were observed by colored with improving potassium heptaiodobismuthate method, and two prunosus blotches, four dark prunosus spots were delected by concentrated sulfuric acid and formaldehyde-concentrated sulfuric acid method, respectively. However, no chromogenic spots were found by colored with nitric acid as well with ninhydrin. The content of alkaloids in Bozhou Peony Paeonia lactiflora was about 0.079%. Conclusion Bozhou Peony Paeonia lactiflora contained alkaloid, and may including aconitum alkaloids.