中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
10期
44-45,46
,共3页
三圣汤%固体汤剂%高效液相色谱法%松脂醇二葡萄糖苷%绿原酸
三聖湯%固體湯劑%高效液相色譜法%鬆脂醇二葡萄糖苷%綠原痠
삼골탕%고체탕제%고효액상색보법%송지순이포도당감%록원산
Sam Soup%solid decoction%HPLC%pinoresinol diglucoside%chlorogenic acid
目的建立同时测定三圣汤固体汤剂中松脂醇二葡萄糖苷和绿原酸含量的高效液相色谱方法。方法采用 Ultimate XB-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.4%冰醋酸为流动相,梯度洗脱,流速1.0 mL/min,检测波长277 nm,柱温40℃。结果样品中松脂醇二葡萄糖苷的线性范围为5.0～50.0μg,r＝0.9999(n＝6)；绿原酸的线性范围为5.4～54.0μg,r＝0.9996(n＝6)。二者的平均加样回收率分别为98.75%、101.11%,RSD分别为1.39%、2.69%。结论所建立的方法准确、可靠。三圣汤固体汤剂中松脂醇二葡萄糖苷和绿原酸含量高于三圣汤汤剂。
目的建立同時測定三聖湯固體湯劑中鬆脂醇二葡萄糖苷和綠原痠含量的高效液相色譜方法。方法採用 Ultimate XB-C18(250 mm×4.6 mm,5μm)色譜柱,以乙腈-0.4%冰醋痠為流動相,梯度洗脫,流速1.0 mL/min,檢測波長277 nm,柱溫40℃。結果樣品中鬆脂醇二葡萄糖苷的線性範圍為5.0～50.0μg,r＝0.9999(n＝6)；綠原痠的線性範圍為5.4～54.0μg,r＝0.9996(n＝6)。二者的平均加樣迴收率分彆為98.75%、101.11%,RSD分彆為1.39%、2.69%。結論所建立的方法準確、可靠。三聖湯固體湯劑中鬆脂醇二葡萄糖苷和綠原痠含量高于三聖湯湯劑。
목적건립동시측정삼골탕고체탕제중송지순이포도당감화록원산함량적고효액상색보방법。방법채용 Ultimate XB-C18(250 mm×4.6 mm,5μm)색보주,이을정-0.4%빙작산위류동상,제도세탈,류속1.0 mL/min,검측파장277 nm,주온40℃。결과양품중송지순이포도당감적선성범위위5.0～50.0μg,r＝0.9999(n＝6)；록원산적선성범위위5.4～54.0μg,r＝0.9996(n＝6)。이자적평균가양회수솔분별위98.75%、101.11%,RSD분별위1.39%、2.69%。결론소건립적방법준학、가고。삼골탕고체탕제중송지순이포도당감화록원산함량고우삼골탕탕제。
Objection To estabish a HPLC methold for determining the contents of pinoresinol diglucoside and chlorogenic acid in Sam Soup and its solid decoction. Methods Chromatographic column was Ultimate XB-C18 reversed-phase chromatography column (250 mm×4.6 mm, 5 μm). Mobile phase was acetonitrile-0.4% glacial acetic acid with gradient elutim. The flow rate was 1.0 mL/min, detection wavelength was 277 nm, and column temperature was 40 ℃. Results The pinoresinol diglucoside and showed good linear relationship in the range of 5.0-50.0 μg, r =0.999 9 (n =6), and chlorogenic acid showed linear relationship in the range of 5.4-54.0 μg, r=0.999 6 (n=6). The average recoveries of pinoresinol diglucoside and chlorogenic acid were 98.75% (RSD=1.39%) and 101.11% (RSD=2.69%), respectively. Conclusion The established method was accurate and reliable. The contents of pinoresinol diglucoside and chlorogenic acid in solid decoction are higher than traditional decoction of Sam Soup.
