CAJ | 학술논문

目的观察电针血清对β淀粉样蛋白(Aβ)诱导的原代大鼠海马神经元的保护作用。方法通过Aβ1-40脑内注射建立阿尔茨海默病大鼠模型,予以电针治疗后,留取各组血清。Aβ25-35处理原代培养海马神经元建立体外神经元损伤模型,实验分为正常组、模型组和电针血清组,通过MTT法检测神经元的增殖,采用TUNEL染色法检测神经元的凋亡,通过免疫细胞化学染色检测Caspase-3的表达。结果 MTT检测结果发现,经Aβ处理后,细胞活力显著下降。而与模型组比较,电针血清组细胞增殖能力明显增强(P＜0.01)；TUNEL 染色结果显示,与模型组比较,电针血清组凋亡细胞数目明显减少(P＜0.01)；经Aβ处理48 h后,Caspase-3表达水平明显升高,电针血清组Caspase-3阳性细胞数目较模型组显著减少(P＜0.01)。结论电针血清能促进海马神经元的增殖,降低其 Caspase-3的表达,对抗Aβ的神经毒性,减少神经元的凋亡。
목적관찰전침혈청대β정분양단백(Aβ)유도적원대대서해마신경원적보호작용。방법통과Aβ1-40뇌내주사건립아이자해묵병대서모형,여이전침치료후,류취각조혈청。Aβ25-35처리원대배양해마신경원건입체외신경원손상모형,실험분위정상조、모형조화전침혈청조,통과MTT법검측신경원적증식,채용TUNEL염색법검측신경원적조망,통과면역세포화학염색검측Caspase-3적표체。결과 MTT검측결과발현,경Aβ처리후,세포활력현저하강。이여모형조비교,전침혈청조세포증식능력명현증강(P＜0.01)；TUNEL 염색결과현시,여모형조비교,전침혈청조조망세포수목명현감소(P＜0.01)；경Aβ처리48 h후,Caspase-3표체수평명현승고,전침혈청조Caspase-3양성세포수목교모형조현저감소(P＜0.01)。결론전침혈청능촉진해마신경원적증식,강저기 Caspase-3적표체,대항Aβ적신경독성,감소신경원적조망。
Objective To explore the protective effects of electro-acupuncture (EA) serum onβ-amyloid protein (Aβ) induced primary rat hippcampal neurons. Methods The rat models of Alzheimer's disease were established by intracerebral injection of Aβ1-40. After treated them with EA, the serum was harvested. Primary cultured hippocampal neurons were treated with Aβ25-35 to establish neuronal damage model in vitro, and divided into normal group, model group and EA serum group. The proliferation of neurons was detected by MTT test. Neuronal apoptosis was examined by TUNEL staining, and expression of cysteine aspartic acid proteases-3 (Caspase-3) was detected by immunocytochemical staining. Results MTT test showed that the cell viability was significantly decreased after treated with Aβ. While compared with the model group, cell proliferation of EA serum group was significantly enhanced (P<0.01). TUNEL staining showed that the number of apoptotic cells in EA serum group decreased significantly compared with the model group (P<0.01). After 48 h of Aβ treatment, Caspase-3 expression levels were significantly elevated. However, compared with the model group, the number of Caspase-3 positive cells in EA serum group was significantly reduced (P<0.01). Conclusion The EA serum could promote the proliferation of hippocampal neurons, reduce the expression of Caspase-3, counteract the neurotoxicity of β-amyloid protein, and reduce neuronal apoptosis.