CAJ | 학술논문

目的观察艾烟对快速老化模型小鼠(SAMP8)脑内氨基酸类神经递质的影响,探讨艾烟的抗衰老作用效应及机制。方法将70只SAMP8随机分为模型组和6个艾烟干预组。另外选取10只正常老化小鼠(SAMR1)作为正常组。艾烟干预分为低、中、高3个浓度,干预时间分别为15 min和30 min,每日干预1次,共28 d。采用高效液相色谱法测定各组小鼠脑内谷氨酸(Glu)、天冬氨酸(Asp)、γ-氨基丁酸(GABA)含量。结果模型组小鼠脑内Asp和Glu含量高于正常组,GABA低于正常组。艾烟干预各组小鼠脑内Asp和Glu含量均低于模型组,GABA含量高于模型组,或与模型组相当。不同浓度、时间艾烟干预对Asp和Glu的影响无差异。中浓度30 min的艾烟干预可最大程度提高SAMP8脑内GABA。结论艾烟干预可降低SAMP8脑内过高的Glu、Asp含量,升高过低的GABA含量,通过调整脑内氨基酸的异常代谢达到抗衰老的效应。
목적관찰애연대쾌속노화모형소서(SAMP8)뇌내안기산류신경체질적영향,탐토애연적항쇠로작용효응급궤제。방법장70지SAMP8수궤분위모형조화6개애연간예조。령외선취10지정상노화소서(SAMR1)작위정상조。애연간예분위저、중、고3개농도,간예시간분별위15 min화30 min,매일간예1차,공28 d。채용고효액상색보법측정각조소서뇌내곡안산(Glu)、천동안산(Asp)、γ-안기정산(GABA)함량。결과모형조소서뇌내Asp화Glu함량고우정상조,GABA저우정상조。애연간예각조소서뇌내Asp화Glu함량균저우모형조,GABA함량고우모형조,혹여모형조상당。불동농도、시간애연간예대Asp화Glu적영향무차이。중농도30 min적애연간예가최대정도제고SAMP8뇌내GABA。결론애연간예가강저SAMP8뇌내과고적Glu、Asp함량,승고과저적GABA함량,통과조정뇌내안기산적이상대사체도항쇠로적효응。
Objective To observe the effect of moxa smoke (Artemisia vulgaris) on amino acids neurotransmitters in the brain of senescence accelerated mouse (SAMP8) and explore the anti-aging effect of moxa smoke. Methods Totally 70 SAMP8 were randomized into one model control group and 6 intervention groups, and 10 SAMR1 mice were used as normal control group. There were low, medium and high concentrations of moxa smoke during intervention. Moxa smoke intervention was performed 15 min/30 min each day for 28 days. High performance liquid chromatographic method was used to determine the levels of Glu, Asp and GABA in the brain of the mice. Results Compared with the normal control group, the levels of Asp and Glu in the model control group were significantly higher, while the GABA was significantly lower. The levels of Asp and Glu in 6 intervention groups were significantly lower than those in the model control group, while GABA was significantly higher than or the same as the model control group. There was no significant difference among different intervention groups in the levels of Asp and Glu, while the 30 min-medium concentration moxa smoke had the most significant effect in increasing the level of GABA. Conclusion Moxa smoke intervention could decrease the high level of Asp and Glu, and increase the low level of GABA in SAMP8. It could achieve the effect of anti-aging through adjusting the abnormal metabolism of amino acids in the brain.