CAJ | 학술논문

目的：筛选制备8-甲氧基补骨脂素（MOP）柔性纳米脂质体的合适方法。方法：根据正交实验设计及预试验，确定基本配方大豆磷脂∶胆固醇∶M O P∶胆酸钠＝20∶10∶3∶10。分别采用逆相蒸发法、乙醚注入法、薄膜法、薄膜-超声法等方法制备M OP柔性纳米脂质体，比较脂质体包封率、平均粒径和Zeta电位。结果：逆相蒸发法制备的脂质体包封率低，粒径大；乙醚注入法包封率低且平均粒径小；薄膜法包封率高，粒径大；薄膜-超声法包封率高，平均粒径适中，且Zeta电位负值较高；超声15min获得的脂质体包封率较高，平均粒径大小合适，Zeta电位的负值也比较高。结论：采用薄膜-超声法，超声时间15 m in是制备补骨脂素柔性纳米脂质体比较适宜的方法。
목적：사선제비8-갑양기보골지소（MOP）유성납미지질체적합괄방법。방법：근거정교실험설계급예시험，학정기본배방대두린지∶담고순∶M O P∶담산납＝20∶10∶3∶10。분별채용역상증발법、을미주입법、박막법、박막-초성법등방법제비M OP유성납미지질체，비교지질체포봉솔、평균립경화Zeta전위。결과：역상증발법제비적지질체포봉솔저，립경대；을미주입법포봉솔저차평균립경소；박막법포봉솔고，립경대；박막-초성법포봉솔고，평균립경괄중，차Zeta전위부치교고；초성15min획득적지질체포봉솔교고，평균립경대소합괄，Zeta전위적부치야비교고。결론：채용박막-초성법，초성시간15 m in시제비보골지소유성납미지질체비교괄의적방법。
Objective:Screening suitable method for preparing flexible nano-liposome of 8- methoxypsor-alen .Methods :According to orthogonal experimental design and pre-test the basic recipe was determined :soybean lecithin :cholesterol:MOP:sodium cholate = 20 :10:3 :10 .MOP flexible nano-liposomes were prepared by using ei-ther reverse-phase evaporation method ,aether infusion method ,film-dispersion or film-dispersion-ultrasound meth-od .Liposomal envelopment efficiency ,the average particle size and zeta potential were compared between different methods .Results :Reverse-phase evaporation method has low envelopment efficiency and large particle size ;aether infusion method has low envelopment efficiency and small particle size ;film-dispersion method has high envelopment efficiency and large particle size ;film-dispersion-ultrasound method has high envelopment efficiency and suitable par-ticle size and zeta potential .Conclusion:The film-ultrasonic method with ultrasonic time of 15 minutes for preparing MOP flexible nano liposome is a more appropriate method .