CAJ | 학술논문

以抗逆突变株Clostridium beijerinckii IB4为出发菌株，通过常压室温等离子体诱变（ ARTP ），刃天青平板初筛，摇瓶发酵复筛，筛选出1株高抗逆高丁比的突变菌株C.beijerinckii IT111。发酵结果表明：该突变菌株利用多种C源时均展现其高丁醇比的特性，以玉米芯酸解糖液为C源时，溶剂产量达到10.5 g/L，丁醇8.0 g/L，丁醇比高达76％。抑制物抗逆性测试结果显示：糠醛和酸类对C.beijerinckii发酵影响较小，酚类物质对C.beijerinckii抑制作用较强，其中以香草醛为最。综上所述，C.beijerinckii IT111是1株极具潜力的利用木质纤维原料制备丁醇的菌株。
이항역돌변주Clostridium beijerinckii IB4위출발균주，통과상압실온등리자체유변（ ARTP ），인천청평판초사，요병발효복사，사선출1주고항역고정비적돌변균주C.beijerinckii IT111。발효결과표명：해돌변균주이용다충C원시균전현기고정순비적특성，이옥미심산해당액위C원시，용제산량체도10.5 g/L，정순8.0 g/L，정순비고체76％。억제물항역성측시결과현시：강철화산류대C.beijerinckii발효영향교소，분류물질대C.beijerinckii억제작용교강，기중이향초철위최。종상소술，C.beijerinckii IT111시1주겁구잠력적이용목질섬유원료제비정순적균주。
Clostridium beijerinckii IT111,a tolerance mutant strain with high proportions of butanol,was obtained by atmospheric and room temperature plasma ( ARTP ) and high-throughput screening.Fermentation results showed that the mutant had a high butanol proportions using a variety of carbon sources.When non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid ( SAHHC) was used as a substrate,total solvent( ABE) and butanol production of 10.5 g/L and 8.0 g/L were obtained,respectively.The butanol proportion was up to 76%.The results of toxicity evaluation of model inhibitors showed that acetates,furfural and 5-HMF were not inhibitory to ABE production,while phenolic compounds were potent inhibitors of growth and ABE production, especially vanillin.C.beijerinckii IT111 was a promising strain for ABE production from lignocellulosic materials.