中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
3期
508-510
,共3页
Beclin 1%微管相关蛋白轻链3%胃癌
Beclin 1%微管相關蛋白輕鏈3%胃癌
Beclin 1%미관상관단백경련3%위암
Beclin 1%Microtubule-associated protein 1 light chain 3%Gastric cancer
目的 检测自噬相关基因Beelin 1和微管相关蛋白轻链3(LC3)在胃癌组织中的表达,探讨其与胃癌临床病理参数及患者预后的关系.方法 应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法分别检测96例胃癌石蜡组织和癌旁组织中Beclin 1和LC3的蛋白表达水平.应用实时定量聚合酶链反应(Real-time PCR)和Western blot检测27例新鲜胃癌组织和相应癌旁组织中mRNA和蛋白的表达.结果 新鲜胃癌组织中Beclin l mRNA(ΔCt:5.90±1.52)及蛋白(1.060±0.112)的表达水平较癌旁组织(ΔCt:7.50±1.22;0.964±0.130)高,两者之间差异有统计学意义(=4.87,t=4.643,P＜0.05).新鲜胃癌组织中LC3 mRNA(ΔCt:5.00±1.34)及蛋白(1.115±0.111)的表达水平较癌旁组织(ΔCt:6.62±1.51;1.002±0.134)高,两者之间差异有统计学意义(t=5.90,t=5.653,P＜ 0.05).在胃癌石蜡组织中,Beclin l蛋白和LC3表达阳性率(67.7％、64.6％)均高于癌旁组织(45.8％、40.6％),差异有统计学意义(P＜0.01).Beclin 1蛋白和LC3均与肿瘤的分化程度、浸润深度、淋巴结转移以及临床分期有关(P＜0.05).Beclin 1和LC3蛋白阳性表达患者的5年生存率明显低于阴性表达者(P＜0.01).结论 Beclin 1和LC3蛋白表达增高与胃癌的发生、发展相关,提示其发挥着癌基因的作用.
目的 檢測自噬相關基因Beelin 1和微管相關蛋白輕鏈3(LC3)在胃癌組織中的錶達,探討其與胃癌臨床病理參數及患者預後的關繫.方法 應用免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)法分彆檢測96例胃癌石蠟組織和癌徬組織中Beclin 1和LC3的蛋白錶達水平.應用實時定量聚閤酶鏈反應(Real-time PCR)和Western blot檢測27例新鮮胃癌組織和相應癌徬組織中mRNA和蛋白的錶達.結果 新鮮胃癌組織中Beclin l mRNA(ΔCt:5.90±1.52)及蛋白(1.060±0.112)的錶達水平較癌徬組織(ΔCt:7.50±1.22;0.964±0.130)高,兩者之間差異有統計學意義(=4.87,t=4.643,P＜0.05).新鮮胃癌組織中LC3 mRNA(ΔCt:5.00±1.34)及蛋白(1.115±0.111)的錶達水平較癌徬組織(ΔCt:6.62±1.51;1.002±0.134)高,兩者之間差異有統計學意義(t=5.90,t=5.653,P＜ 0.05).在胃癌石蠟組織中,Beclin l蛋白和LC3錶達暘性率(67.7％、64.6％)均高于癌徬組織(45.8％、40.6％),差異有統計學意義(P＜0.01).Beclin 1蛋白和LC3均與腫瘤的分化程度、浸潤深度、淋巴結轉移以及臨床分期有關(P＜0.05).Beclin 1和LC3蛋白暘性錶達患者的5年生存率明顯低于陰性錶達者(P＜0.01).結論 Beclin 1和LC3蛋白錶達增高與胃癌的髮生、髮展相關,提示其髮揮著癌基因的作用.
목적 검측자서상관기인Beelin 1화미관상관단백경련3(LC3)재위암조직중적표체,탐토기여위암림상병리삼수급환자예후적관계.방법 응용면역조직화학련매균항생물소단백-과양화물매(SP)법분별검측96례위암석사조직화암방조직중Beclin 1화LC3적단백표체수평.응용실시정량취합매련반응(Real-time PCR)화Western blot검측27례신선위암조직화상응암방조직중mRNA화단백적표체.결과 신선위암조직중Beclin l mRNA(ΔCt:5.90±1.52)급단백(1.060±0.112)적표체수평교암방조직(ΔCt:7.50±1.22;0.964±0.130)고,량자지간차이유통계학의의(=4.87,t=4.643,P＜0.05).신선위암조직중LC3 mRNA(ΔCt:5.00±1.34)급단백(1.115±0.111)적표체수평교암방조직(ΔCt:6.62±1.51;1.002±0.134)고,량자지간차이유통계학의의(t=5.90,t=5.653,P＜ 0.05).재위암석사조직중,Beclin l단백화LC3표체양성솔(67.7％、64.6％)균고우암방조직(45.8％、40.6％),차이유통계학의의(P＜0.01).Beclin 1단백화LC3균여종류적분화정도、침윤심도、림파결전이이급림상분기유관(P＜0.05).Beclin 1화LC3단백양성표체환자적5년생존솔명현저우음성표체자(P＜0.01).결론 Beclin 1화LC3단백표체증고여위암적발생、발전상관,제시기발휘착암기인적작용.
Objective To detect the expression of Autophagy-related Genes Beclin 1 and microtubule-associated protein 1 light chain 3 (LC3) protein in gastric cancer and to analyze the correlations with clinical pathological parameters and prognosis of patients with gastric carcinoma.Methods The expression of Beclin 1 and LC3 proteins were examined in 96 paraffin blocks of the gastric cancer and adjacent normal gastric mucosa by Immunohistochemical SP method.The expression levels of mRNA and proteins in 27 fresh gastric carcinoma samples and their corresponding adjacent noncancerous tissues were examined by real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting.Results The level of Beclin 1 mRNA (ΔCt:5.90 ± 1.52) and Beclin 1 protein (1.060 ±0.112) in fresh gastric carcinoma tissues was significantly higher than that (ΔCt:7.50 ± 1.22; 0.964 ±0.130) in their adjacent normal tissues (t =4.87,t =4.643,P ＜ 0.05).The level 3 of LC3 mRNA (Δ Ct:5.00 ± 1.34) and LC3 protein (1.115 ±0.111) in fresh gastric carcinoma tissues was significantly higher than that (ΔCt:6.62 ±1.51 ; 1.002 ± 0.134) in their adjacent normal tissues (t =5.90,t =5.653,P ＜ 0.05).The positive percentage of Beclin 1 proteins and LC3 proteins in paraffin blocks of the gastric cancer was 67.7％ and 64.6％,which was significantly higher than that 45.8％ and 40.6％ in tumor adjacent tissue (P ＜0.01).Beclin 1 and LC3 protein expressions were correlated with pathological differentiation,invasion depth,lymph node metastasis,tumor stage (P ＜0.05).There was a significant correlation between Beclin 1 and LC3 protein in gastric cancer (r =0.420,P ＜ 0.01).The patients with positive expression of Beclin 1 and LC3 proteins had a significantly lower 5-year survival rate than those with negative expression (P ＜ 0.01).Conclusion Up-regulation of Beclin 1 and LC3 correlate with the genesis and development of gastric carcinoma.
