中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
9期
617-620
,共4页
红斑狼疮,系统性%Blimp1%B细胞成熟抗原%浆细胞%小鼠,近交MRLlpr
紅斑狼瘡,繫統性%Blimp1%B細胞成熟抗原%漿細胞%小鼠,近交MRLlpr
홍반랑창,계통성%Blimp1%B세포성숙항원%장세포%소서,근교MRLlpr
Lupus erythematosus,systemic%Blimp1%B-cell maturation antigen%Plasma cell%Mice,inbred MRL lpr
目的 探讨Blimp1在MRL/lpr狼疮鼠中的表达水平,为靶向浆细胞治疗SLE提供思路.方法 用EMSA和CHIP证实Blimp1对B细胞成熟抗原(BCMA)的调控作用,并用实时定量PCR检测MRL/lpr狼疮鼠与正常鼠脾脏和淋巴结中Blimp1 mRNA的表达差异.结果 Blimp1能与BCMA基因启动子相结合.狼疮鼠组脾脏Blimp1mRNA的相对表达水平高于正常对照组Blimp1 mRNA的相对表达水平(Z=2.609,P＜0.01),狼疮鼠组淋巴结中Blimp1的相对表达水平也高于正常对照组Blimp1 mRNA的相对表达水平(Z=3.402,P＜ 0.01),差异有统计学意义.结论 BCMA可能是Blimp1的靶基因,Blimp1可直接调控BCMA表达,在维持浆细胞存活及抗体分泌中发挥作用.
目的 探討Blimp1在MRL/lpr狼瘡鼠中的錶達水平,為靶嚮漿細胞治療SLE提供思路.方法 用EMSA和CHIP證實Blimp1對B細胞成熟抗原(BCMA)的調控作用,併用實時定量PCR檢測MRL/lpr狼瘡鼠與正常鼠脾髒和淋巴結中Blimp1 mRNA的錶達差異.結果 Blimp1能與BCMA基因啟動子相結閤.狼瘡鼠組脾髒Blimp1mRNA的相對錶達水平高于正常對照組Blimp1 mRNA的相對錶達水平(Z=2.609,P＜0.01),狼瘡鼠組淋巴結中Blimp1的相對錶達水平也高于正常對照組Blimp1 mRNA的相對錶達水平(Z=3.402,P＜ 0.01),差異有統計學意義.結論 BCMA可能是Blimp1的靶基因,Blimp1可直接調控BCMA錶達,在維持漿細胞存活及抗體分泌中髮揮作用.
목적 탐토Blimp1재MRL/lpr랑창서중적표체수평,위파향장세포치료SLE제공사로.방법 용EMSA화CHIP증실Blimp1대B세포성숙항원(BCMA)적조공작용,병용실시정량PCR검측MRL/lpr랑창서여정상서비장화림파결중Blimp1 mRNA적표체차이.결과 Blimp1능여BCMA기인계동자상결합.랑창서조비장Blimp1mRNA적상대표체수평고우정상대조조Blimp1 mRNA적상대표체수평(Z=2.609,P＜0.01),랑창서조림파결중Blimp1적상대표체수평야고우정상대조조Blimp1 mRNA적상대표체수평(Z=3.402,P＜ 0.01),차이유통계학의의.결론 BCMA가능시Blimp1적파기인,Blimp1가직접조공BCMA표체,재유지장세포존활급항체분비중발휘작용.
Objective To detect the expression of Blimp1 gene in MRL/lpr mice,so as to provide new ideas for plasma cell-targeting therapy of systemic lupus erythematosus (SLE).Methods Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation analysis (ChIP) were performed to evaluate the regulatory effect of Blimp1 on B cell maturation antigen (BCMA),and fluorescence-based quantitative PCR was carried out to detect the expression of Blimp1 mRNA in spleen and lymph node tissue of MRL/lpr mice and normal control mice.The intergroup difference in Blimp l mRNA expression was assessed by rank sum test.Results Blimp1 could bind to the BCMA gene promoter.Increased Blimp1 mRNA expression was observed in both the spleen and lymph node tissue of MRL/lpr mice compared with the normal control mice (Z =2.609,3.402,respectively,both P ＜ 0.01).Conclusions BCMA appears to be the target gene of Blimp1,and Blimp1 may plays a certain role in the maintenance of plasma cell survival and antibody production via directly regulating BCMA gene expression.
