CAJ | 학술논문

目的：检测大鼠脊髓损伤（spinal cord injury，SCI）后血-脊髓屏障（blood spinal cord barrier, BSCB）紧密连接蛋白claudin-5表达变化。方法120只成年雄性SD大鼠随机分为空白组（60只）和损伤组（60只），损伤组参照改良Allen’s法[2]建立脊髓损伤动物模型。建模后不同时间点（6h、12h、1d、3d、5d、7d）各组随机取5只大鼠检测BSCB通透性，取胸段脊髓损伤区组织经RT-PCR、Western blot方法检测claudin-5表达。结果损伤组制模成功率81.7%；损伤组EB含量随时间变化逐渐升高，在损伤后第3天达峰值（0.9435±0.0813）μg/g，此后逐渐降低（P均＜0.05），各时间点损伤组EB含量较空白组显著增高（P均＜0.05)；损伤组claudin-5 mRNA表达随时间变化逐渐减少，在损伤后第3天表达最低（2.871±0.527），此后逐渐升高（P均＜0.05），各时间点损伤组claudin-5 mRNA表达均较空白组显著降低（P均＜0.05)；损伤组claudin-5表达随时间变化逐渐减少，第3天达最低（0.072±0.008），之后逐渐增加（P均＜0.05），各时间点损伤组claudin-5表达均较空白组显著降低（P均＜0.05）。结论大鼠SCI后可能通过影响claudin-5的表达改变BSCB通透性，影响脊髓继发损伤的病理生理过程。
목적：검측대서척수손상（spinal cord injury，SCI）후혈-척수병장（blood spinal cord barrier, BSCB）긴밀련접단백claudin-5표체변화。방법120지성년웅성SD대서수궤분위공백조（60지）화손상조（60지），손상조삼조개량Allen’s법[2]건립척수손상동물모형。건모후불동시간점（6h、12h、1d、3d、5d、7d）각조수궤취5지대서검측BSCB통투성，취흉단척수손상구조직경RT-PCR、Western blot방법검측claudin-5표체。결과손상조제모성공솔81.7%；손상조EB함량수시간변화축점승고，재손상후제3천체봉치（0.9435±0.0813）μg/g，차후축점강저（P균＜0.05），각시간점손상조EB함량교공백조현저증고（P균＜0.05)；손상조claudin-5 mRNA표체수시간변화축점감소，재손상후제3천표체최저（2.871±0.527），차후축점승고（P균＜0.05），각시간점손상조claudin-5 mRNA표체균교공백조현저강저（P균＜0.05)；손상조claudin-5표체수시간변화축점감소，제3천체최저（0.072±0.008），지후축점증가（P균＜0.05），각시간점손상조claudin-5표체균교공백조현저강저（P균＜0.05）。결론대서SCI후가능통과영향claudin-5적표체개변BSCB통투성，영향척수계발손상적병리생리과정。
Objective To examine the expression of tight junction protein claudin-5 in blood-spinal cord barrier (BSCB) after spinal cord injury about rat. Methods One hundred-twenty adult male SD rats were randomly divided into blank group (60) and injured group (60). The animal model of spinal cord injury was established using modified Allen method. The expression of claudin-5 in BSCB was examined at 6 h, 12 h, 1 d, 3 d, 5 d and 7 d (five rats per time point). Western blot and RT_PCR were used to detect protein and mRNA expression levels of claudin-5, respectively. Results The success rate of spinal cord injury molding was 81.7%. In injured group, EB content increased gradually over time, reached the peak at the third day(0.9435 ± 0.0813)μg/g and then reduced gradually (P<0.05), EB content was signifi-cantly higher in injured group than in blank group. Claudin-5 mRNA expression in injured group reduced gradually over time and reached the lowest point at the third day(2.871 ± 0.527)and then increased gradually(P<0.05). Claudin-5 mRNA expression was significantly lower in injured group than in blank group(P<0.05). Claudin-5 protein expression in injured group reduced gradually over time, reached the lowest at the third day(0.072 ±0.008)and then increased gradually (P<0.05). Claudin- 5 protein expression was significantly lower in injured group than in blank group(P<0.05). Con-clusions The alteration of claudin-5 expression after SCI may lead to the permeability of BSCB, which may in turn con-tribute to the secondary spinal cord injury.