化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2013年
9期
1283-1285
,共3页
奈韦拉平%铬天青S%电荷转移反应
奈韋拉平%鉻天青S%電荷轉移反應
내위랍평%락천청S%전하전이반응
nevirapineoride%chromeazurol s%charge transfer reaction
确定了奈韦拉平与铬天青S的电荷转移反应条件，同时建立了测定奈韦拉平的分光光度法。奈韦拉平与铬天青S在水和丙酮混合溶剂中发生电荷转移反应，常温下反应30min后可比色。电荷转移络合物的最大吸收波长是578 nm，表观摩尔吸光系数为1.78×104 L· mol-1· cm-1。奈韦拉平质量浓度在1～16μg· mL-1范围内服从比耳定律，相关系数为0.9993。当奈韦拉平浓度为6μg· mL-1时，六次测定结果的相对标准偏差为1.61％。测得荷移络合物的组成和稳定常数分别为1∶1和1.73×104。本方法用于测定制剂中奈韦拉平的含量，结果与文献方法一致，回收率在98.0％以上。
確定瞭奈韋拉平與鉻天青S的電荷轉移反應條件，同時建立瞭測定奈韋拉平的分光光度法。奈韋拉平與鉻天青S在水和丙酮混閤溶劑中髮生電荷轉移反應，常溫下反應30min後可比色。電荷轉移絡閤物的最大吸收波長是578 nm，錶觀摩爾吸光繫數為1.78×104 L· mol-1· cm-1。奈韋拉平質量濃度在1～16μg· mL-1範圍內服從比耳定律，相關繫數為0.9993。噹奈韋拉平濃度為6μg· mL-1時，六次測定結果的相對標準偏差為1.61％。測得荷移絡閤物的組成和穩定常數分彆為1∶1和1.73×104。本方法用于測定製劑中奈韋拉平的含量，結果與文獻方法一緻，迴收率在98.0％以上。
학정료내위랍평여락천청S적전하전이반응조건，동시건립료측정내위랍평적분광광도법。내위랍평여락천청S재수화병동혼합용제중발생전하전이반응，상온하반응30min후가비색。전하전이락합물적최대흡수파장시578 nm，표관마이흡광계수위1.78×104 L· mol-1· cm-1。내위랍평질량농도재1～16μg· mL-1범위내복종비이정률，상관계수위0.9993。당내위랍평농도위6μg· mL-1시，륙차측정결과적상대표준편차위1.61％。측득하이락합물적조성화은정상수분별위1∶1화1.73×104。본방법용우측정제제중내위랍평적함량，결과여문헌방법일치，회수솔재98.0％이상。
The charge transfer reaction in water-acetone between nevirapineoride as donor and chromeazurol S as acceptor was inves -tigated by spectrophotometry .The experimental optimum conditions were established .The apparent molar absorptivity of charge transfer complex is 1.78×104L· mol-1· cm-1 at 578 nm.Beer’s Law is obeyed in the range of 1~16μg· mL-1 of nevirapineoride with correlation coefficient of 0.9993.The relative standard deviation is 1.61%(n=6)when the concentration of nevirapineoride is 6μg· mL-1 .The average recovery was over 98.0%.The composition ratio and stability constant is 1∶1 and 1.73×104 of the charge transfer complex in water-acetone between nevirapineoride and chromeazurol S respectively .The proposed method had been applied to determine the content of nevirapineoride in preparations with satisfactory results .
