CAJ | 학술논문

用固相法合成了乙酰阿魏酰酪氨酰酪氨酸（ F-Tyr-Tyr），并用ESI-MS、IR、NMR对其结构进行表征。应用紫外光谱法、荧光光谱法研究了F-Tyr-Tyr与小牛胸腺DNA（ ctDNA）的相互作用。结果表明，F-Tyr-Tyr与ctDNA相互作用后体系的紫外吸收呈减色效应，且F-Tyr-Tyr加入后，DNA-EB体系的荧光强度减弱。根据Stern -Volmer方程求出F-Tyr-Tyr-DNA-EB体系的猝灭常数为K SV＝6.28×103L· mol -1，猝灭方式为静态猝灭，推测F-Tyr-Tyr与ctDNA 的相互作用模式主要是静电结合。
용고상법합성료을선아위선락안선락안산（ F-Tyr-Tyr），병용ESI-MS、IR、NMR대기결구진행표정。응용자외광보법、형광광보법연구료F-Tyr-Tyr여소우흉선DNA（ ctDNA）적상호작용。결과표명，F-Tyr-Tyr여ctDNA상호작용후체계적자외흡수정감색효응，차F-Tyr-Tyr가입후，DNA-EB체계적형광강도감약。근거Stern -Volmer방정구출F-Tyr-Tyr-DNA-EB체계적졸멸상수위K SV＝6.28×103L· mol -1，졸멸방식위정태졸멸，추측F-Tyr-Tyr여ctDNA 적상호작용모식주요시정전결합。
Acetylferulic-Tyr-Tyr( F-Tyr-Tyr) was synthesized by solid phase method and characterized by ESI-MS,IR and NMR.The interaction between F-Tyr-Tyr and ctDNA was investigated by ultraviolet spectroscopy and fluorescence spectroscopy.The results showed that the ultraviolet spectrum produced hypochromic effect and the fluorescence had quenching effect with increasing the con -centration of F-Tyr-Tyr.According to the Stern-Volmer equation ,the quenching constant was 6.28 ×10 3 L· mol-1 and the quenching process was suggested as static.From these results,it can be predicted that the interaction between F-Tyr-Tyr and DNA was mainly in electrostatic binding mode.