CAJ | 학술논문

目的研究机械通气所致急性肺损伤对大鼠肺组织中核内激素受体（Nr1d1）表达的影响，探讨其在机械通气肺损伤中可能的作用机制。方法72只大鼠分成4个时间点（1：00 AM，7：00 AM，1：00 PM，7：00 PM），每个时间点随机分成3组（FB组、LV组、HV组），通气3 h后，提取肺组织RNA，应用大鼠全基因组芯片和荧光定量实时PCR检测机械通气对Nr1d1表达的影响。结果基因芯片结果显示HV组Nr1d1表达下调，荧光定量实时PCR结果与芯片结果吻合；余弦分析Nr1d1基因在FB组具有节律性（P<0.05），余未见明显节律性。结论 Nr1d1表达随潮气量的增大而节律消失；Nr1d1表达下降与机械通气所致急性肺损伤密切相关。
목적연구궤계통기소치급성폐손상대대서폐조직중핵내격소수체（Nr1d1）표체적영향，탐토기재궤계통기폐손상중가능적작용궤제。방법72지대서분성4개시간점（1：00 AM，7：00 AM，1：00 PM，7：00 PM），매개시간점수궤분성3조（FB조、LV조、HV조），통기3 h후，제취폐조직RNA，응용대서전기인조심편화형광정량실시PCR검측궤계통기대Nr1d1표체적영향。결과기인심편결과현시HV조Nr1d1표체하조，형광정량실시PCR결과여심편결과문합；여현분석Nr1d1기인재FB조구유절률성（P<0.05），여미견명현절률성。결론 Nr1d1표체수조기량적증대이절률소실；Nr1d1표체하강여궤계통기소치급성폐손상밀절상관。
Objective To investigate the changes in the expression of nuclear receptor subfamily1 (Nr1d1) in the lungs of rats with ventilation-induced lung injury and explore the molecular mechanism. Methods Seventy-two rats were randomly divided into 4 groups for observation at 4 time points (1:00 AM, 7:00 AM, 1:00 PM and 7:00 PM), and each group was further divided into 3 subgroups (Group FB, Group LV and Group HV). After ventilation for 3 h, the rats were sacrificed and lung tissue samples were obtained for testing the expression of Nr1d1 using gene chip technology and RT-PCR. Results Nr1d1 gene expression was down-regulated in HV group as shown by gene chip RT-PCR data. Cosine analysis showed that Nr1d1 expression exhibited a rhythmicity in FB group (P<0.05). Conclusion The rhythmicity of Nr1d1 expression disappears as the tidal volume the increase. Diminished Nr1d1 expression is closely correlated with ventilation-induced lung injury in rats.