CAJ | 학술논문

目的：检测血红素氧合酶-1（heme oxygenase，HO -1）在良、恶性结直肠组织中的表达情况，探讨其在结直肠肿瘤发生发展中的作用。方法：采用免疫组化的方法检测118例结直肠癌、17例结直肠炎性息肉及20例结直肠癌周围正常组织患者的石蜡标本中 HO -1的表达情况，使用图像分析系统测定阳性细胞的平均光密度值。结果：结直肠癌组织 HO -1的平均光密度值随着肿瘤分化程度提高而上升，结直肠癌组织 HO -1的平均光密度值高于炎性息肉及直肠癌周围良性组织，差异有统计学意义（P ﹤0.05）。结直肠癌组织 HO -1的平均光密度值与性别、TNM 分期及淋巴结转移无关。结论：HO -1在结直肠癌中呈现高表达，与结直肠良性病变及癌变存在相关性，可能与结直肠癌的发生、发展及预后有关联。
목적：검측혈홍소양합매-1（heme oxygenase，HO -1）재량、악성결직장조직중적표체정황，탐토기재결직장종류발생발전중적작용。방법：채용면역조화적방법검측118례결직장암、17례결직장염성식육급20례결직장암주위정상조직환자적석사표본중 HO -1적표체정황，사용도상분석계통측정양성세포적평균광밀도치。결과：결직장암조직 HO -1적평균광밀도치수착종류분화정도제고이상승，결직장암조직 HO -1적평균광밀도치고우염성식육급직장암주위량성조직，차이유통계학의의（P ﹤0.05）。결직장암조직 HO -1적평균광밀도치여성별、TNM 분기급림파결전이무관。결론：HO -1재결직장암중정현고표체，여결직장량성병변급암변존재상관성，가능여결직장암적발생、발전급예후유관련。
To study the expression of heme oxygenase - 1(HO - 1)in benign and malignant colorectal tissues and explore its function in colorectal carcinoma development. Methods:The expression of HO - 1 in 118 color-ectal carcinoma,17 polyps and 20 non - tumoral adjacent tissue was detected by immunohistochemistry method,and the mean optical density of positive cells were measured by image analysis technology. Results:The mean optical den-sity of HO - 1 increased in colorectal carcinoma with the degree of carcinoma differentiation rise. The mean optical density of HO - 1was higher in colorectal carcinoma than that in polyps and colorectal carcinoma surrounding normal tissue,there was statistical significance in HO - 1 expression between groups. The mean optical density of HO - 1 had no correlation with gender,TNM stage and lymph node metastasis. Conclusion:HO - 1 was highly expressed in color-ectal carcinoma tissue and associated with malignant colorectal tissue. HO - 1 is probably related with occurrence,de-velopment and prognosis of colorectal carcinoma.