CAJ | 학술논문

目的：探讨胚胎冷冻技术对小鼠2-4细胞胚胎细胞骨架结构的影响。方法用抗骨架蛋白 actin 的单克隆抗体进行免疫染色，激光共聚焦显微镜观察经过慢速冷冻小鼠2-细胞、4-细胞胚胎的细胞骨架结构的状况。结果以丙二醇为冷冻保护剂，采用慢速冷冻-快速解冻的方式冷冻小鼠2-细胞、4-细胞胚胎对胚胎细胞骨架结构的分布特点没有明显影响，荧光强度定量分析表明冷冻胚胎荧光强度与未冷冻胚胎的荧光强度相比，差异无统计学意义（P >0.05）。结论冷冻对小鼠2-细胞和4-细胞胚胎的细胞骨架结构没有明显影响。
목적：탐토배태냉동기술대소서2-4세포배태세포골가결구적영향。방법용항골가단백 actin 적단극륭항체진행면역염색，격광공취초현미경관찰경과만속냉동소서2-세포、4-세포배태적세포골가결구적상황。결과이병이순위냉동보호제，채용만속냉동-쾌속해동적방식냉동소서2-세포、4-세포배태대배태세포골가결구적분포특점몰유명현영향，형광강도정량분석표명냉동배태형광강도여미냉동배태적형광강도상비，차이무통계학의의（P >0.05）。결론냉동대소서2-세포화4-세포배태적세포골가결구몰유명현영향。
Objective TO investigate the effects Of cryOPreservatiOn On the cytOskeletOn Of 2 - 4cell mOuse embryOs. Methods ImmunOhistOchemical staining was used tO assess the status Of skeletOn PrOtein actin Of mOuse embryOs. The cytOskeletOn Of mOuse cryOPreserved embryOs was Observed by laser - cOnfOcal micrOscOPe. Results The slOwing - freezing and fast - thawing PrOtOcOl with PROH as cryOPrOtectant had little effect On the distributing characteristic Of cytOskeletOn Of 2 - cell,4 - cell mOuse embryOs,and had little effect On the fluOrescent intensity by fluOrescence quantitative analysis,with nO significant differences(P > 0. 05). Conclusion CryOPreservatiOn had little effect On the cytOskeletOn Of 2 - 4 cell mOuse embryOs.