CAJ | 학술논문

目的：探讨月光花豆乙醇提取物( CABE)对急性肺损伤( ALI)大鼠的保护作用。方法大鼠尾静脉注射脂多糖5 mg·kg-1复制 ALI 模型,免疫组化法测定大鼠肺组织中核因子-кBp65( NF-кBp65)的表达,酶联免疫吸附( ELISA)法测定肺组织白细胞介素-1β( IL-1β)的含量,同时观察肺组织病理形态、肺组织的湿干质量比例( W/D)和肺组织髓过氧化物酶(MPO)的活性。结果模型大鼠和CABE治疗大鼠肺组织W/D分别为5.05±0.24,4.35±0.21；IL-1β含量分别为(320.60±19.73),(294.42±13.95) pg·mL-1；MPO活性分别为(1.31±0.18),(1.04±0.18) U·g-1；与模型大鼠比较, CABE治疗大鼠肺组织损伤减轻, IL-1β含量减少,肺组织W/D和MPO活性降低, NF-кBp65表达降低( P<0.01)。结论CABE对脂多糖诱导的ALI大鼠具有明显的保护作用,干扰NF-кB途径和抑制IL-1β的生成可能是其作用机制之一。
목적：탐토월광화두을순제취물( CABE)대급성폐손상( ALI)대서적보호작용。방법대서미정맥주사지다당5 mg·kg-1복제 ALI 모형,면역조화법측정대서폐조직중핵인자-кBp65( NF-кBp65)적표체,매련면역흡부( ELISA)법측정폐조직백세포개소-1β( IL-1β)적함량,동시관찰폐조직병리형태、폐조직적습간질량비례( W/D)화폐조직수과양화물매(MPO)적활성。결과모형대서화CABE치료대서폐조직W/D분별위5.05±0.24,4.35±0.21；IL-1β함량분별위(320.60±19.73),(294.42±13.95) pg·mL-1；MPO활성분별위(1.31±0.18),(1.04±0.18) U·g-1；여모형대서비교, CABE치료대서폐조직손상감경, IL-1β함량감소,폐조직W/D화MPO활성강저, NF-кBp65표체강저( P<0.01)。결론CABE대지다당유도적ALI대서구유명현적보호작용,간우NF-кB도경화억제IL-1β적생성가능시기작용궤제지일。
Objective To investigate the protective effects of ethanol extract of calonyction acculeatum beans( CABE) on rats with acute lung injury ( ALI ) . Methods The ALI model was induced via intravenous injection with 5 mg · kg-1 lipopolysaccharide(LPS). The expression of nuclear factor-кB p65(NF-кB65) was determind by immunohistochemistry(IHC), and the content of interleukin-1β( IL-1β) in the lung tissue was detected by enzyme-linked immunosorbent assay ( ELISA ) . Meanwhile,lung tissue histopathology,the wet-dry weight ratio of lung and the activity of myeloperoxidase(MPO) in the lung tissues were observed. Results Compared with the model group, CABE lessened the lung injury, decreased IL-1β level, reduced the lung dry-wet weight ratio and activity of MPO,and down-regulated the expression of NF-кB/p65(P<0. 05). Conclusion CABE has remarkedly protective effect on ALI induced by LPS in rats. One of the mechanisms may be related to reducing the secretion of IL-1β and interfering with the activity of NF-кB.