CAJ | 학술논문

该研究旨在建立一种快速、灵敏及准确的口蹄疫病毒(FMDV)鉴定与分型的RT-PCR检测技术平台，为临床中有效准确地检测口蹄疫病毒提供有力的支持。根据NCBI中公布的FMDV序列(GenBank：JF749861.1)，设计FMDV通用检测引物FP1/FP2；根据NCBI中公布的FMDV O型、A型和Asia I型序列，经过序列分析比对后，分别设计FMDV分型引物FO1/FO2、FA1/FA2和FAS1/FAS2。提取FMDV RNA后，利用随机引物扩增得到FMDV全cDNA，利用设计的引物进行PCR扩增及PCR反应条件的优化。结果表明：该研究建立的RT-PCR检测方法具有很好的特异性、敏感性和可重复性。成功建立了FMDV O型、A型和Asia I型检测及分型方法，为口蹄疫疾病的临床检测、流行病学相关调查及针对性的疫苗的制备奠定基础
해연구지재건립일충쾌속、령민급준학적구제역병독(FMDV)감정여분형적RT-PCR검측기술평태，위림상중유효준학지검측구제역병독제공유력적지지。근거NCBI중공포적FMDV서렬(GenBank：JF749861.1)，설계FMDV통용검측인물FP1/FP2；근거NCBI중공포적FMDV O형、A형화Asia I형서렬，경과서렬분석비대후，분별설계FMDV분형인물FO1/FO2、FA1/FA2화FAS1/FAS2。제취FMDV RNA후，이용수궤인물확증득도FMDV전cDNA，이용설계적인물진행PCR확증급PCR반응조건적우화。결과표명：해연구건립적RT-PCR검측방법구유흔호적특이성、민감성화가중복성。성공건립료FMDV O형、A형화Asia I형검측급분형방법，위구제역질병적림상검측、류행병학상관조사급침대성적역묘적제비전정기출
To establish a rapid,sensitive and accurate foot-and-mouth disease virus(FMDV)identification and genotyping of RT-PCR detection technology,and provide strong support for the effective and accurate detection of foot-and-mouth disease virus in clinical,we designed the FMDV general detection primer FP1/FP2 according to the the NCBI published in FMDV sequence(GenBank:JF749861.1),and designed the FMDV genotyping primers FO1/FO2,FA1/FA2 and FAS1/FAS2 according to the FMDV type O,type A and type Asia I sequence from NCBI after sequence analysis.After extraction of the FMDV RNA,we got the FMDV cDNA using random primers,and used cDNA as the template for PCR amplification and optimized the PCR reaction conditions.The results showed that RT-PCR genotyping methods established in this study had a good specificity,sensitivity and reproducibility.The results indicated that the RT-PCR genotyping method for foot-and-mouth disease virus Type O,A and AsiaIhad been successfully established,and laid the foundation for the clinical detection epidemiological investigation and targeted vaccine preparation of FMD disease in clinical research.