CAJ | 학술논문

为了建立高效液相色谱法测定尼卡巴嗪的含量，采用Waters X-Bridge C18（150 mm ×4．6 mm，5μm）色谱柱，乙腈-水（用磷酸调pH至3．0）为流动相，梯度洗脱，流速为1．0 mL·min-1，检测波长0～5 min为297 nm，5 min开始为350 nm，柱温30℃，进样体积为20μL。2-羟基-4，6-二甲基嘧啶（HDP）的回归方程为：y＝23452x-456，相关系数r＝0．99999，4，4’-二硝基均二苯脲（ DNC）的回归方程为：y ＝102388x ＋5296，相关系数r＝0．9999，即HDP在0．29～29．12μg／mL范围内具有良好的线性， DNC在0．71～70．90μg／mL范围内具有良好的线性。该方法简便、准确、灵敏度高、重复性好，可以通过同时测定尼卡巴嗪中HDP和DNC来计算尼卡巴嗪的含量。
위료건립고효액상색보법측정니잡파진적함량，채용Waters X-Bridge C18（150 mm ×4．6 mm，5μm）색보주，을정-수（용린산조pH지3．0）위류동상，제도세탈，류속위1．0 mL·min-1，검측파장0～5 min위297 nm，5 min개시위350 nm，주온30℃，진양체적위20μL。2-간기-4，6-이갑기밀정（HDP）적회귀방정위：y＝23452x-456，상관계수r＝0．99999，4，4’-이초기균이분뇨（ DNC）적회귀방정위：y ＝102388x ＋5296，상관계수r＝0．9999，즉HDP재0．29～29．12μg／mL범위내구유량호적선성， DNC재0．71～70．90μg／mL범위내구유량호적선성。해방법간편、준학、령민도고、중복성호，가이통과동시측정니잡파진중HDP화DNC래계산니잡파진적함량。
To develop the method of HPLC for determination of nicarbazine. The liquid chromatography separation was carried on XBridge C18 column (150 mm×4.6 mm,5μm) with acetonitrile-water(pH was adjusted to 3.0 by H3PO4) as mobile phase under the column temperature 30℃.The detection wavelength was 297 nm at 0~5 min, and 350 nm after 5 min, and the injection quantity was 20 μL . The equation of HDP was y = 23452x - 456, r=0.99999;The equation of DNC was y =102388x + 5296,r=0.9999, the linear range of HDP and DNC were 0.29~29.12 μg/mL and 0. 71~70. 90 μg/mL respectively. The method was simple , accuracy, and with high sensitivity.The content of nicarbazine can be calculated by determining HDP and DNC at the same time.