湖南中医药大学学报
湖南中醫藥大學學報
호남중의약대학학보
JOURNAL OF HUNAN COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
5期
18-22
,共5页
类风湿性关节炎%Ⅱ型胶原性关节炎%黄芪虫藤饮%大鼠
類風濕性關節炎%Ⅱ型膠原性關節炎%黃芪蟲籐飲%大鼠
류풍습성관절염%Ⅱ형효원성관절염%황기충등음%대서
rheumatoid arthritis%type Ⅱ collagen-induced arthritis%Astragalus bug cane drink%SD rats
目的:观察黄芪虫藤饮对Ⅱ型胶原性关节炎(collagen-induced arthritis, CIA)大鼠的抗炎作用及对大鼠血清血沉(ESR)、类风湿因子(RF)的影响,进一步探讨该药的作用机制。方法将80只SD大鼠随机分为空白组10只,其余建立大鼠CIA模型,将造模成功的大鼠随机分为模型组、甲氨喋呤组、正清风痛宁组、黄芪虫藤饮高、中、低剂量组(下简称高、中、低剂量组)各组10只,分别给药,观察各组大鼠关节炎指数(Arthrits, AI)积分,踝关节病理切片变化,取大鼠血清检测ESR、RF含量。结果 AI积分在给药2、3、4周后,各给药组大鼠与模型组比较,差异有统计学意义(P<0.05或P<0.01),其中各时间点中、低剂量组与高剂量组比较,差异有统计学意义(P<0.05或P<0.01);给药2、3周后高剂量组与正清风痛宁组比较,差异有统计学意义(P<0.05)。各给药组与模型组比较,ESR、RF的含量均降低,差异有统计学意义(P<0.01),中、低剂量组与高剂量组比较,差异有统计学意义(P<0.01);高剂量组RF含量下降比甲氨喋呤组、正清风痛宁组明显,差异有统计学意义(P<0.01)。结论黄芪虫藤饮可降低CIA大鼠关节症状评分,抑制血清ESR、RF升高,对CIA大鼠具有较好的抗炎、免疫调节作用。
目的:觀察黃芪蟲籐飲對Ⅱ型膠原性關節炎(collagen-induced arthritis, CIA)大鼠的抗炎作用及對大鼠血清血沉(ESR)、類風濕因子(RF)的影響,進一步探討該藥的作用機製。方法將80隻SD大鼠隨機分為空白組10隻,其餘建立大鼠CIA模型,將造模成功的大鼠隨機分為模型組、甲氨喋呤組、正清風痛寧組、黃芪蟲籐飲高、中、低劑量組(下簡稱高、中、低劑量組)各組10隻,分彆給藥,觀察各組大鼠關節炎指數(Arthrits, AI)積分,踝關節病理切片變化,取大鼠血清檢測ESR、RF含量。結果 AI積分在給藥2、3、4週後,各給藥組大鼠與模型組比較,差異有統計學意義(P<0.05或P<0.01),其中各時間點中、低劑量組與高劑量組比較,差異有統計學意義(P<0.05或P<0.01);給藥2、3週後高劑量組與正清風痛寧組比較,差異有統計學意義(P<0.05)。各給藥組與模型組比較,ESR、RF的含量均降低,差異有統計學意義(P<0.01),中、低劑量組與高劑量組比較,差異有統計學意義(P<0.01);高劑量組RF含量下降比甲氨喋呤組、正清風痛寧組明顯,差異有統計學意義(P<0.01)。結論黃芪蟲籐飲可降低CIA大鼠關節癥狀評分,抑製血清ESR、RF升高,對CIA大鼠具有較好的抗炎、免疫調節作用。
목적:관찰황기충등음대Ⅱ형효원성관절염(collagen-induced arthritis, CIA)대서적항염작용급대대서혈청혈침(ESR)、류풍습인자(RF)적영향,진일보탐토해약적작용궤제。방법장80지SD대서수궤분위공백조10지,기여건립대서CIA모형,장조모성공적대서수궤분위모형조、갑안첩령조、정청풍통저조、황기충등음고、중、저제량조(하간칭고、중、저제량조)각조10지,분별급약,관찰각조대서관절염지수(Arthrits, AI)적분,과관절병리절편변화,취대서혈청검측ESR、RF함량。결과 AI적분재급약2、3、4주후,각급약조대서여모형조비교,차이유통계학의의(P<0.05혹P<0.01),기중각시간점중、저제량조여고제량조비교,차이유통계학의의(P<0.05혹P<0.01);급약2、3주후고제량조여정청풍통저조비교,차이유통계학의의(P<0.05)。각급약조여모형조비교,ESR、RF적함량균강저,차이유통계학의의(P<0.01),중、저제량조여고제량조비교,차이유통계학의의(P<0.01);고제량조RF함량하강비갑안첩령조、정청풍통저조명현,차이유통계학의의(P<0.01)。결론황기충등음가강저CIA대서관절증상평분,억제혈청ESR、RF승고,대CIA대서구유교호적항염、면역조절작용。
Objective To observe the anti-inflammatory effects of Astragalus bug cane drink on typeⅡcollagen-induced arthritis (CIA) rats and the effects on serum erythrocyte sedimentation rate (ESR) and rheumatoid factor (RF), to further explore the mechanism of action of the drug. Methods SD rats were divided into control group (n=10), and CIA rats, CIA rats were then randomly divided in-to model group, methotrexate group, Zhengqingfengtongning group, Astragalus bug cane drink high, medium and low dose group (hereinafter referred to as high, medium and low dose group) . Arthritis index (AI), serum ESR and RF were evaluated; pathological changes of ankle joints were examined. Results AI of treat-ment groups was significantly different from CIA model group in 2, 3, 4 weeks, AI of low-dose group and medium-dose group at each time point was significantly different from high-dose group (P<0.05 or P<0.01). AI of high-dose group was significantly different from Zhengqingfengtongning group after treating 2 to 3 weeks. (P<0.05). ESR and RF of treatment group was significantly lower in treat ment groups compared with the model group ( P<0 . 01 ) , the difference of low-dose group and medium-dose group compared with the high-dose group was statistically significant (P<0.01). RF was significantly reduced with high-dose group than MTX group and Zhengqingfengtongning group (P<0.01), high-dose group was superior to the methotrexate group and Zhengqingfengtongning groups. Conclusion Astragalus bug cane drink can reduce joint symptoms score, suppress the elevation of serum ESR and RF in type Ⅱ collagen-induced arthritis rats, with anti-inflammatory and im-munomodulatory effects.