CAJ | 학술논문

通过紫外诱变提高嗜酸乳杆菌的耐酸耐胆盐能力。通过正交试验确定了诱变参数：20 W紫外灯，菌液稀释至OD6000.65左右，照射60 s，照射距离为30.5 cm，此条件的致死率为90.5%。诱变处理后的菌体在模拟人肠道胆汁盐浓度（0.3%）以及pH=2的选择性培养基中处理不同时间后，活菌计数，最终筛选出具有耐胆盐能力显著增强的突变体L.a.abr。在含0.3%胆盐和pH为2的选择性培养基中培养1.5、2.0、2.5 h时的存活率分别为19.57%、10.87%和4.78%，而出发菌株的相应存活率分别为3.68%、1%、0.23%。
통과자외유변제고기산유간균적내산내담염능력。통과정교시험학정료유변삼수：20 W자외등，균액희석지OD6000.65좌우，조사60 s，조사거리위30.5 cm，차조건적치사솔위90.5%。유변처리후적균체재모의인장도담즙염농도（0.3%）이급pH=2적선택성배양기중처리불동시간후，활균계수，최종사선출구유내담염능력현저증강적돌변체L.a.abr。재함0.3%담염화pH위2적선택성배양기중배양1.5、2.0、2.5 h시적존활솔분별위19.57%、10.87%화4.78%，이출발균주적상응존활솔분별위3.68%、1%、0.23%。
The study was designed to enhance the acid and bile salt tolerant ability of Lactobacillus acidophilus by UV mutation. Parameters with a lethal rate 90.5%for UV mutation determined by orthogonal test were 20 W UV, bacteria was diluted to around OD600 0.65, illuminate at a distance of 30.5 cm for 60 s. The mutant was screened on the selective medium with a simulated human intestinal bile salt concentration of 0.3%and pH=2 . Cell counting was carried out after incubation for different periods. L.a.abr was screened out that with an enhanced ability to tolerate high bile salt concentration with a survival rate of 19.57%,10.87%and 4.78%after incubated for 1.5 h,2.0 h and 2.5 h respectively,while under the same situation, the starter bacteria was 3.68 %,1%and 0.23%.