CAJ | 학술논문

建立高效液相色谱法快速测定荞麦冷面中芦丁含量的分析方法。采用甲醇-水（体积比1∶1，含0.1%硼砂保护剂）超声提取冷面中的芦丁，在Shimpack VP-ODS色谱柱（250 mm×4.6 mm，5μm）上，以甲醇-1%冰乙酸（40∶60，体积比）为流动相分离，紫外检测器257 mm波长检测。采用外标法定量，该方法在0.25μg/mL～50.0μg/mL浓度范围内有良好的线性关系，相关系数R2为0.9997，加标回收率均在95%以上，相对标准偏差不大于2.4%，方法最低检出限为1.25 mg/kg。该方法具有快速、准确、灵敏度高，样品前处理简便易行等优点，为荞麦冷面的生产加工和检测提供了参考。
건립고효액상색보법쾌속측정교맥랭면중호정함량적분석방법。채용갑순-수（체적비1∶1，함0.1%붕사보호제）초성제취랭면중적호정，재Shimpack VP-ODS색보주（250 mm×4.6 mm，5μm）상，이갑순-1%빙을산（40∶60，체적비）위류동상분리，자외검측기257 mm파장검측。채용외표법정량，해방법재0.25μg/mL～50.0μg/mL농도범위내유량호적선성관계，상관계수R2위0.9997，가표회수솔균재95%이상，상대표준편차불대우2.4%，방법최저검출한위1.25 mg/kg。해방법구유쾌속、준학、령민도고，양품전처리간편역행등우점，위교맥랭면적생산가공화검측제공료삼고。
To determine the content of Rutin in buckwheat noodles using high performance liquid chromatography. The sample was ultrasonic extracted with methanol-water (1∶1,volume ratio, concluding 0.1%Borax protective agent), and separated in Shimpack VP-ODS column (250 mm×4.6 mm,5μm) using methanol-0.1%glacial acetic acid (40∶60,volume ratio) as mobile phase, and determined at 257 nm by UV detector. the calibration curve of Rutin in buckwheat noodles at the concentration range of 0.25μg/mL-50.0μg/mL was good linear (R2 0.999 7). The recoveries of Rutin in buckwheat noodles were above 95%and the relative standard deviation was not more than 2.4%. The lowest detection limit was 1.25 mg/kg. This method had the advantage of speedy, exactness, high sensitivity and simple preparation, which provided the foundation for production and processing and detection of buckwheat noodles.