CAJ | 학술논문

应用胶体金免疫层析技术，研制出一种快速检测T-2毒素的方法。采用柠檬酸三钠还原法制备20 nm胶体金，标记抗T-2毒素多克隆抗体并喷于玻璃纤维上，T-2毒素偶联抗原和羊抗兔二抗分别结合于硝酸纤维素膜上，经过试纸条层析条件优化，依次将样本垫、金标结合释放垫、硝酸纤维膜和吸水纸组装切割成胶体金试纸条。测试结果表明T-2毒素快速检测试纸条的灵敏度为50μg/L，检测时间为10 min，批内和批间重复性好，保存期为3个月。使用简单方便，非常适合现场快速检测T-2毒素。
응용효체금면역층석기술，연제출일충쾌속검측T-2독소적방법。채용저몽산삼납환원법제비20 nm효체금，표기항T-2독소다극륭항체병분우파리섬유상，T-2독소우련항원화양항토이항분별결합우초산섬유소막상，경과시지조층석조건우화，의차장양본점、금표결합석방점、초산섬유막화흡수지조장절할성효체금시지조。측시결과표명T-2독소쾌속검측시지조적령민도위50μg/L，검측시간위10 min，비내화비간중복성호，보존기위3개월。사용간단방편，비상괄합현장쾌속검측T-2독소。
To establish a rapid assay for detection of T-2 toxin, gold immunochromatography assay (GICA) was used. The polyclonal antibody towards T-2 toxin marked by 20 nm Colloidal gold was sprayed onto the glass fiber. T-2 HS-OVA and goat anti-rabbit IgG antibodies were jet-positioned onto nitrocellulose membrane. After the conditions of chromatographic were optimized, sample pad, gold conjugate pad, nitrocellulose membrane and absorbent paper were assembled, respectively. The results showed that the visual detection limit for the rapid assay of T-2 toxin was 50μg/L, detection time is within 10 minutes and repeatability of the strip was good. The shelf-life is three month. The assay is simply used for the rapid detection of T-2 toxin on-site.