CAJ | 학술논문

目的观察原花青素及其与抗炎活性成分（积雪草苷、姜黄素、茶多酚）的组合物对氧化低密度脂蛋白（ox- LDL）刺激巨噬细胞形成泡沫细胞过程中炎症细胞因子和细胞内胆固醇含量的变化。方法以体外培养的小鼠单核巨噬细胞系RAW264.7细胞为材料和ox- LDL刺激RAW264.7细胞24h形成泡沫细胞为模型，RT- PCR方法检测细胞因子IL-1β和TNF-αmRNA水平，试剂盒测定细胞内游离胆固醇和总胆固醇的含量。结果原花青素及其与积雪草苷、姜黄素、茶多酚组合物可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值，抑制泡沫细胞的形成，显著抑制泡沫细胞的IL-1β和TNF-αmRNA表达，其中原花青素与姜黄素的组合物作用最强。结论原花青素组合物通过降低细胞内胆固醇酯的含量，减轻细胞泡沫化程度和减轻细胞的炎症反应起到抗动脉粥样硬化的作用。
목적관찰원화청소급기여항염활성성분（적설초감、강황소、다다분）적조합물대양화저밀도지단백（ox- LDL）자격거서세포형성포말세포과정중염증세포인자화세포내담고순함량적변화。방법이체외배양적소서단핵거서세포계RAW264.7세포위재료화ox- LDL자격RAW264.7세포24h형성포말세포위모형，RT- PCR방법검측세포인자IL-1β화TNF-αmRNA수평，시제합측정세포내유리담고순화총담고순적함량。결과원화청소급기여적설초감、강황소、다다분조합물가명현강저포말세포내적담고순지여총담고순적비치，억제포말세포적형성，현저억제포말세포적IL-1β화TNF-αmRNA표체，기중원화청소여강황소적조합물작용최강。결론원화청소조합물통과강저세포내담고순지적함량，감경세포포말화정도화감경세포적염증반응기도항동맥죽양경화적작용。
Objective To study the effect of Proanthocyanidins and its composition with Anti- inflammatory active ingredi-ents such as Asiaticoside, Curcumin or tea polyphenols on cytokine levels and intracellular cholesterol during foam cellformation induced by oxidized low density lipoprotein (ox- LDL). Methods Mouse monocyte- macrophage cell line RAW264.7 cells were cultured in vitro and the cells were stimulated by ox- LDL for 24h to transform foam cells. IL- 1βmRNA and TNF- α mRNA was evaluated by real time PCR. An enzymatic colorimetric assay was used to determine intracellular cholesterol. Results Proantho-cyanidins and its composition with Asiaticoside, Curcumin or tea polyphenols can significantly reduced the cholesterol esters and the ratio of total cholesterol in the foam cells, inhibite foam cells formation and expression of IL- 1βmRNA and TNF- α mRNA . The composition of Proanthocyanidins and curcumin had the strongest effect. Conclusion Proanthocyanidins and its composi-tion play a role of anti- atherosclerosis by reducing intracellular cholesterol ester content, foam cells formation and the inflamma-tory response.